%0 Journal Article
%T Genetic characterization and fine mapping of a recessive genic male-sterile gene in flowering Chinese cabbage (Brassica rapa var. parachinensis).
%A Wu Z
%A Yang Y
%A Li T
%A Shen Z
%A Zhou X
%A Zhang Y
%J 3 Biotech
%V 14
%N 6
%D 2024 Jun
%M 38779526
%F 2.893
%R 10.1007/s13205-024-04005-7
%X Brassica vegetables exhibit pronounced heterosis; nevertheless, investigations on fertility-related genes are scarce. The present study scrutinized a recessive genic male-sterile line, 7-3A, capable of generating a completely sterile population, holding significant promise for flowering Chinese cabbage breeding. By whole-genome resequencing of sterile and fertile plants, the male-sterile gene was confined to approximately 185 kb on chromosome A07, situated between markers C719 and NP10 in Brassica rapa var. Chiifu-401. Notably, substantial structural variation was identified within this region across diverse Brassica rapa reference genomes. Despite discernible expression level disparities of a homologous gene, Bnams4b, between male sterile and fertile plants, no sequence divergence was detected. Further elucidation is required to pinpoint a novel sterile gene within the candidate interval. This investigation contributes to the advancement of both the molecular-assisted breeding scheme for flowering Chinese cabbage and the comprehension of male sterility mechanisms.<BR><B>UNASSIGNED: </B>The online version contains supplementary material available at 10.1007/s13205-024-04005-7.