Abstract：
A Gram-stain-negative, aerobic, non-motile, catalase- and oxidase-positive, pale orange, rod-shaped strain EF6T, was isolated from a natural wetland reserve in Hebei province, China. The strain grew at 25-37 °C (optimum, 30 °C), pH 5-9 (optimum, pH 7), and in the presence of 1.0-4.0% (w/v) NaCl (optimum, 2%). A phylogenetic analysis based on 16S rRNA gene sequence revealed that strain EF6T belongs to the genus Paracoccus, and the closest members were Paracoccus shandongensis wg2T with 98.1% similarity, Paracoccus fontiphilus MVW-1 T (97.9%), Paracoccus everestensis S8-55 T (97.7%), Paracoccus subflavus GY0581T (97.6%), Paracoccus sediminis CMB17T (97.3%), Paracoccus caeni MJ17T (97.0%), and Paracoccus angustae E6T (97.0%). The genome size of strain EF6T was 4.88 Mb, and the DNA G + C content was 65.3%. The digital DNA-DNA hybridization, average nucleotide identity, and average amino acid identity values between strain EF6T and the reference strains were all below the threshold limit for species delineation (< 32.8%, < 88.0%, and < 86.7%, respectively). The major fatty acids (≥ 5.0%) were summed feature 8 (86.3%, C18:1 ω6c and/or C18:1 ω7c) and C18:1 (5.0%) and the only isoprenoid quinone was Q-10. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids, five unidentified phospholipids, and an unidentified aminolipid. Strain EF6T displays notable resistance to benzoate and selenite, with higher tolerance levels (25 g/L for benzoate and 150 mM for selenite) compared to the closely related species. Genomic analysis identified six benzoate resistance genes (acdA, pcaF, fadA, pcaC, purB, and catA) and twenty selenite resistance and reduction-related genes (iscR, ssuB, ssuD, selA, selD and so on). Additionally, EF6T possesses unique genes (catA, ssuB, and ssuC) absent in the closely related species for benzoate and selenite resistance. Its robust resistance to benzoate and selenite, coupled with its genomic makeup, make EF6T a promising candidate for the remediation of both organic and inorganic pollutants. It is worth noting that the specific resistance phenotypes described above were not reported in other novel species in Paracoccus. Based on the results of biochemical, physiological, phylogenetic, and chemotaxonomic analyses, combined with comparisons of the 16S rRNA gene sequence and the whole genome sequence, strain EF6T is considered to represent a novel species of the genus Paracoccus within the family Rhodobacteraceae, for which the name Paracoccus benzoatiresistens sp. nov. is proposed. The type strain is EF6T (= GDMCC 1.3400 T = JCM 35642 T = MCCC 1K08702T).
摘要：
革兰氏染色阴性，有氧,不活动，过氧化氢酶和氧化酶阳性，淡橙色,杆状应变EF6T，是从河北省的天然湿地保护区中分离出来的，中国。该菌株在25-37°C生长(最佳，30°C),pH5-9（最佳，pH7),并且在1.0-4.0%(w/v)NaCl存在下(最佳，2%)。基于16SrRNA基因序列的系统发育分析表明，菌株EF6T属于副球菌属，最接近的成员是shandongensiswg2T副球菌，相似性为98.1%，梅毒副球菌MVW-1T（97.9%），副球菌S8-55T（97.7%），副球菌GY0581T（97.6%），床旁球菌CMB17T(97.3%),副球菌MJ17T（97.0％），和骨旁球菌E6T（97.0％）。菌株EF6T的基因组大小为4.88Mb，DNAG+C含量为65.3%。数字DNA-DNA杂交,平均核苷酸同一性，菌株EF6T和参考菌株之间的平均氨基酸同一性值均低于物种划分的阈值极限（<32.8％，<88.0%，<86.7%，分别)。主要脂肪酸(≥5.0%)为特征8(86.3%，C18：1ω6c和/或C18：1ω7c）和C18：1（5.0％），唯一的类异戊二烯醌是Q-10。极性脂质由二磷脂酰甘油组成，磷脂酰甘油，两种身份不明的糖脂，五种身份不明的磷脂，和一种身份不明的氨基脂质.菌株EF6T对苯甲酸酯和亚硒酸盐表现出明显的抗性，与密切相关的物种相比，具有更高的耐受性水平（苯甲酸盐25g/L，亚硒酸盐150mM）。基因组分析确定了六个苯甲酸盐抗性基因(acdA，pcaF,fada,pcac,purb,和catA)和二十个亚硒酸盐抗性和还原相关基因(iscR，ssub,susud,sela,selD等等)。此外,EF6T具有独特的基因(catA，ssub,和ssuC）在苯甲酸盐和亚硒酸盐抗性密切相关的物种中不存在。其强大的抗苯甲酸盐和亚硒酸盐，再加上它的基因组组成，使EF6T成为修复有机和无机污染物的有希望的候选者。值得注意的是，在副球菌的其他新物种中未报道上述特定的抗性表型。根据生化结果,生理,系统发育，和化学分类学分析，结合16SrRNA基因序列和全基因组序列的比较，菌株EF6T被认为代表了红杆菌科内副球菌属的一种新物种，名称为苯甲酸副球菌抗性sp。11月。是提议的。菌株类型为EF6T(=GDMCC1.3400T=JCM35642T=MCCC1K08702T)。
