PDF(Pubmed)
Abstract：
Functional bowel disorders (FBD) have a major potential to degrade the standards of public life. Juniperus oxycedrus L. (J. oxycedrus) (Cupressaceae) has been described as a plant used in traditional medicine as an antidiarrheal medication. The present study is the first to obtain information on the antispasmodic and antidiarrheic effects of J. oxycedrus aqueous extract through in vitro and in vivo studies. An aqueous extract of J. oxycedrus (AEJO) was extracted by decoctioning air-dried aerial sections of the plant. Antispasmodic activity was tested in an isolated jejunum segment of rats exposed to cumulative doses of drogue extract. The antidiarrheic activity was tested using diarrhea caused by castor oil, a transit study of the small intestine, and castor oil-induced enteropooling assays in mice. In the jejunum of rats, the AEJO (0.1, 0.3 and 1 mg/ml) diminished the maximum tone induced by low K+ (25 mM), while it exhibited a weak inhibitory effect on high K+ (75 mM) with an IC50=0.49 ± 0.01 mg/ml and IC50=2.65 ± 0.16 mg/ml, respectively. In the contractions induced by CCh (10-6 M), AEJO diminished the maximum tone, similar to that induced by low K+ (25 mM). with an IC50=0.45 ± 0.02 mg/ml. The inhibitory effect of AEJO on low K+ induced contractions was significantly diminished in the presence of glibenclamide (GB) (0.3 µM) and 4-aminopyrimidine (4-AP) (100 µM), with IC50 values of 1.84 ± 0.09 mg/ml. and 1.63 ± 0.16 mg/ml, respectively). The demonstrated inhibitory effect was similar to that produced by a non-competitive antagonist acting on cholinergic receptors and calcium channels. In castor oil-induced diarrhea in mice, AEJO (100, 200, and 400 mg/kg) caused an extension of the latency time, a reduced defecation frequency, and a decrease in the amount of wet feces compared to the untreated group (distilled water). Moreover, it showed a significant anti-motility effect and reduced the amount of fluid accumulated in the intestinal lumen at all tested doses. These findings support the conventional use of Juniperus oxycedrus L. as a remedy for gastrointestinal diseases.
摘要：
功能性肠病（FBD）具有降低公共生活标准的主要潜力。朱尼佩鲁斯·奥克塞勒斯·L(J.oxycedrus）（柏科）已被描述为在传统医学中用作止泻药的植物。本研究是第一个通过体外和体内研究获得有关氧化松柏水提物的抗痉挛和抗腹泻作用的信息。通过煎煮植物的风干地上部分来提取氧化刺柏（AEJO）的水提取物。在暴露于累积剂量的牙髓提取物的大鼠的离体空肠段中测试了抗痉挛活性。使用蓖麻油引起的腹泻测试了抗腹泻活性，小肠的运输研究，和蓖麻油诱导的小鼠肠汇集试验。在老鼠的空肠，AEJO（0.1，0.3和1mg/ml）降低了低K（25mM）诱导的最大音调，尽管它对高K（75mM）的抑制作用较弱，IC50=0.49±0.01mg/ml，IC50=2.65±0.16mg/ml，分别。在CCh（10-6M）诱导的收缩中，AEJO减弱了最大音调，与低K+(25mM)诱导的相似。IC50=0.45±0.02mg/ml。在格列本脲（GB）（0.3µM）和4-氨基嘧啶（4-AP）（100µM）存在下，AEJO对低钾诱导的收缩的抑制作用显着减弱，IC50值为1.84±0.09mg/ml。和1.63±0.16毫克/毫升，分别)。所证明的抑制作用与作用于胆碱能受体和钙通道的非竞争性拮抗剂产生的抑制作用相似。在蓖麻油诱导的小鼠腹泻中，AEJO（100、200和400mg/kg）导致潜伏期延长，减少排便频率，与未处理组(蒸馏水)相比,湿粪便的量减少。此外，在所有测试剂量下，它都显示出显着的抗运动作用，并减少了肠腔中积聚的液体量。这些发现支持JuniperusoxyceedrusL.的常规用途作为胃肠道疾病的补救措施。
