Abstract：
BACKGROUND: The aim of this study was to establish features of inflammation in histologically normal gallbladders with gallstones and compare the expression of inflammatory markers in acutely and chronically inflamed gallbladders.
METHODS: Immunohistochemistry was performed on formalin-fixed paraffin-embedded gallbladders for tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-2R, and substance p in three groups: Group I (n = 60) chronic cholecystitis, Group II (n = 57) acute cholecystitis and Group III (n = 45) histologically normal gallbladders with gallstones. Expression was quantified using the H-scoring system.
RESULTS: Median, interquartile range expression of mucosal IL-2R in Groups I (2.65, 0.87-7.97) and II (12.30, 6.15-25.55) was significantly increased compared with group III (0.40, 0.10-1.35, p < 0.05). Submucosal IL-2R expression in Groups I (2.0, 1.12-4.95) and II (10.0, 5.95-14.30) was also significantly increased compared with Group III (0.50, 0.15-1.05, p < 0.05). There was no difference in the lymphoid cell IL-6 expression between Groups I (5.95, 1.60-18.15), II (6.10, 1.1-36.15) and III (8.30, 2.60-26.35, p > 0.05). Epithelial IL-6 expression of Group III (8.3, 2.6-26.3) was significantly increased compared with group I (0.5, 0-10.2, p < 0.05) as was epithelial TNF-α expression in Group III (85.0, 70.50-92.0) compared with Groups I (72.50, 45.25.0-85.50, p < 0.05) and II (61.0, 30.0-92.0, p < 0.05). Lymphoid cell Substance P expression in Groups I (1.90, 1.32-2.65) and II (5.62, 2.50-20.8) was significantly increased compared with Group III (1.0,1.0-1.30, p < 0.05). Epithelial cell expression of Substance P in Group III (121.7, 94.6-167.8) was significantly increased compared with Groups I (75.7, 50.6-105.3, p < 0.05) and II (78.9, 43.5-118.5, p < 0.05).
CONCLUSIONS: Histologically normal gallbladders with gallstones exhibited features of inflammation on immunohistochemistry.
METHODS: Immunohistochemistry was performed on formalin-fixed paraffin-embedded gallbladders for tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-2R, and substance p in three groups: Group I (n = 60) chronic cholecystitis, Group II (n = 57) acute cholecystitis and Group III (n = 45) histologically normal gallbladders with gallstones. Expression was quantified using the H-scoring system.
RESULTS: Median, interquartile range expression of mucosal IL-2R in Groups I (2.65, 0.87-7.97) and II (12.30, 6.15-25.55) was significantly increased compared with group III (0.40, 0.10-1.35, p < 0.05). Submucosal IL-2R expression in Groups I (2.0, 1.12-4.95) and II (10.0, 5.95-14.30) was also significantly increased compared with Group III (0.50, 0.15-1.05, p < 0.05). There was no difference in the lymphoid cell IL-6 expression between Groups I (5.95, 1.60-18.15), II (6.10, 1.1-36.15) and III (8.30, 2.60-26.35, p > 0.05). Epithelial IL-6 expression of Group III (8.3, 2.6-26.3) was significantly increased compared with group I (0.5, 0-10.2, p < 0.05) as was epithelial TNF-α expression in Group III (85.0, 70.50-92.0) compared with Groups I (72.50, 45.25.0-85.50, p < 0.05) and II (61.0, 30.0-92.0, p < 0.05). Lymphoid cell Substance P expression in Groups I (1.90, 1.32-2.65) and II (5.62, 2.50-20.8) was significantly increased compared with Group III (1.0,1.0-1.30, p < 0.05). Epithelial cell expression of Substance P in Group III (121.7, 94.6-167.8) was significantly increased compared with Groups I (75.7, 50.6-105.3, p < 0.05) and II (78.9, 43.5-118.5, p < 0.05).
CONCLUSIONS: Histologically normal gallbladders with gallstones exhibited features of inflammation on immunohistochemistry.
摘要：
背景：本研究的目的是确定组织学正常的胆囊结石患者的炎症特征，并比较急性和慢性发炎的胆囊炎性标志物的表达。
方法：对福尔马林固定石蜡包埋的胆囊进行免疫组织化学，以检测肿瘤坏死因子（TNF）-α，白细胞介素（IL）-6，IL-2R，和P物质三组：I组（n=60）慢性胆囊炎，II组（n=57）急性胆囊炎和III组（n=45）组织学正常的胆囊结石。使用H评分系统定量表达。
结果：中位数，与III组相比，I组（2.65，0.87-7.97）和II组（12.30，6.15-25.55）的粘膜IL-2R的四分位间距表达显着增加（0.40，0.10-1.35，p<0.05）。与III组相比，I组（2.0，1.12-4.95）和II组（10.0，5.95-14.30）的粘膜下IL-2R表达也显着增加（0.50，0.15-1.05，p<0.05）。I组淋巴细胞IL-6表达无差异(5.95,1.60-18.15),Ⅱ(6.10,1.1-36.15)和Ⅲ(8.30,2.60-26.35,p>0.05)。与I组（0.5，0-10.2，p<0.05）相比，III组的上皮IL-6表达（8.3，2.6-26.3）显着增加，III组的上皮TNF-α表达（85.0，70.50-92.0）与I组（72.50，45.25.0-85.50，p<0.05）和II组（61.0，30.0-92.0，p<0.05）。与III组相比，I组（1.90，1.32-2.65）和II组（5.62，2.50-20.8）的淋巴样细胞P物质表达显着增加（1.0，1.0-1.30，p<0.05）。与I组（75.7，50.6-105.3，p<0.05）和II组（78.9，43.5-118.5，p<0.05）相比，III组（121.7，94.6-167.8）中P物质的上皮细胞表达显着增加。
结论：组织学正常的胆囊结石在免疫组织化学上表现出炎症特征。
方法：对福尔马林固定石蜡包埋的胆囊进行免疫组织化学，以检测肿瘤坏死因子（TNF）-α，白细胞介素（IL）-6，IL-2R，和P物质三组：I组（n=60）慢性胆囊炎，II组（n=57）急性胆囊炎和III组（n=45）组织学正常的胆囊结石。使用H评分系统定量表达。
结果：中位数，与III组相比，I组（2.65，0.87-7.97）和II组（12.30，6.15-25.55）的粘膜IL-2R的四分位间距表达显着增加（0.40，0.10-1.35，p<0.05）。与III组相比，I组（2.0，1.12-4.95）和II组（10.0，5.95-14.30）的粘膜下IL-2R表达也显着增加（0.50，0.15-1.05，p<0.05）。I组淋巴细胞IL-6表达无差异(5.95,1.60-18.15),Ⅱ(6.10,1.1-36.15)和Ⅲ(8.30,2.60-26.35,p>0.05)。与I组（0.5，0-10.2，p<0.05）相比，III组的上皮IL-6表达（8.3，2.6-26.3）显着增加，III组的上皮TNF-α表达（85.0，70.50-92.0）与I组（72.50，45.25.0-85.50，p<0.05）和II组（61.0，30.0-92.0，p<0.05）。与III组相比，I组（1.90，1.32-2.65）和II组（5.62，2.50-20.8）的淋巴样细胞P物质表达显着增加（1.0，1.0-1.30，p<0.05）。与I组（75.7，50.6-105.3，p<0.05）和II组（78.9，43.5-118.5，p<0.05）相比，III组（121.7，94.6-167.8）中P物质的上皮细胞表达显着增加。
结论：组织学正常的胆囊结石在免疫组织化学上表现出炎症特征。
