Abstract：
This work aimed to evaluate the effects of 4 selected essential oils on planktonic cells and microbial biofilms of the Staphylococcus aureus strain (MRSA ATCC 33591). The antibacterial activities of the four essential oils Geranium (Pelargonium graveolens), PgEO, Tea Tree (Melaleuca alternifolia) MaEO, Lemon peel (Citrus limon) ClEO and Peppermint (Mentha piperita) MpEO had MICs ranging from 1.56 to 12.5 µl/ml. The evaluation of the antibiofilm activities of the 4 EOs revealed that they had antiadhesive activities against S. aureus MRSA biofilms; the activity reached 60% (the EO of MpEO peppermint at a concentration of 3.12 µl/ml), and the eradication activity was 80% (the EO of PgEO and MpEO at 3.12 µl/ml). The antibiofilm activity of S. aureus has been explained by the binding of several essential oil bioactive molecules to the SarA protein, the main target protein involved in biofilm formation. The synthesis of the virulence factor staphyloxanthin by S. aureus MRSA ATCC 33591 was significantly inhibited in the presence of PgEO at a concentration of MIC/2. This inhibition was explained by the binding of the main PgEO molecules (β-citronellol and geraniol) to the CrTM protein involved in the staphyloxanthin synthesis pathway. There is evidence that these essential oils could be used as potential anti-virulents to control Staphylococcus biofilm formation.
摘要：
这项工作旨在评估4种选择的精油对金黄色葡萄球菌菌株（MRSAATCC33591）的浮游细胞和微生物生物膜的影响。四种香精油天竺葵（Pelargoniumgraveolens）的抗菌活性，PgEO,茶树（互叶白千层）MaEO，柠檬皮（柑橘柠檬）ClEO和薄荷（薄荷胡椒）MpEO的MIC范围为1.56至12.5μl/ml。对4种EOs的抗生物膜活性的评估表明，它们对金黄色葡萄球菌MRSA生物膜具有抗粘附活性;活性达到60％（浓度为3.12μl/ml的MpEO薄荷的EO），根除活性为80％（PgEO和MpEO的EO为3.12μl/ml）。金黄色葡萄球菌的抗生物膜活性已通过几种精油生物活性分子与SarA蛋白的结合来解释。参与生物膜形成的主要靶蛋白。在浓度为MIC/2的PgEO存在下，金黄色葡萄球菌MRSAATCC33591对毒力因子葡萄球菌氧黄质的合成受到显着抑制。这种抑制作用可以通过主要的PgEO分子（β-香茅醇和香叶醇）与参与葡萄黄质合成途径的CrTM蛋白的结合来解释。有证据表明，这些精油可以用作潜在的抗病毒剂来控制葡萄球菌生物膜的形成。
