PDF(Pubmed)
Abstract：
UNASSIGNED: Polycystic ovary syndrome (PCOS) is a common multifactorial and polygenic disorder of the endocrine system, affecting up to 20% of women in reproductive age with a still unknown etiology. Follicular fluid (FF) represents an environment for the normal development of follicles rich in metabolites, hormones and neurotransmitters, but in some instances of PCOS the composition can be different. Vasoactive intestinal peptide (VIP) is an endogenous autonomic neuropeptide involved in follicular atresia, granulosa cell physiology and steroidogenesis.
UNASSIGNED: ELISA assays were performed to measure VIP and estradiol levels in human follicular fluids, while AMH, FSH, LH, estradiol and progesterone in the plasma were quantified by chemiluminescence. UHPLC/QTOF was used to perform the untargeted metabolomic analysis.
UNASSIGNED: Our ELISA and metabolomic results show: i) an increased concentration of VIP in follicular fluid of PCOS patients (n=9) of about 30% with respect to control group (n=10) (132 ± 28 pg/ml versus 103 ± 26 pg/ml, p=0,03) in women undergoing in vitro fertilization (IVF), ii) a linear positive correlation (p=0.05, r=0.45) between VIP concentration and serum Anti-Müllerian Hormone (AMH) concentration and iii) a linear negative correlation between VIP and noradrenaline metabolism. No correlation between VIP and estradiol (E2) concentration in follicular fluid was found. A negative correlation was found between VIP and noradrenaline metabolite 3,4-dihydroxyphenylglycolaldehyde (DOPGAL) in follicular fluids.
UNASSIGNED: VIP concentration in follicular fluids was increased in PCOS patients and a correlation was found with noradrenaline metabolism indicating a possible dysregulation of the sympathetic reflex in the ovarian follicles. The functional role of VIP as noradrenergic modulator in ovarian physiology and PCOS pathophysiology was discussed.
UNASSIGNED: ELISA assays were performed to measure VIP and estradiol levels in human follicular fluids, while AMH, FSH, LH, estradiol and progesterone in the plasma were quantified by chemiluminescence. UHPLC/QTOF was used to perform the untargeted metabolomic analysis.
UNASSIGNED: Our ELISA and metabolomic results show: i) an increased concentration of VIP in follicular fluid of PCOS patients (n=9) of about 30% with respect to control group (n=10) (132 ± 28 pg/ml versus 103 ± 26 pg/ml, p=0,03) in women undergoing in vitro fertilization (IVF), ii) a linear positive correlation (p=0.05, r=0.45) between VIP concentration and serum Anti-Müllerian Hormone (AMH) concentration and iii) a linear negative correlation between VIP and noradrenaline metabolism. No correlation between VIP and estradiol (E2) concentration in follicular fluid was found. A negative correlation was found between VIP and noradrenaline metabolite 3,4-dihydroxyphenylglycolaldehyde (DOPGAL) in follicular fluids.
UNASSIGNED: VIP concentration in follicular fluids was increased in PCOS patients and a correlation was found with noradrenaline metabolism indicating a possible dysregulation of the sympathetic reflex in the ovarian follicles. The functional role of VIP as noradrenergic modulator in ovarian physiology and PCOS pathophysiology was discussed.
摘要：
■多囊卵巢综合征（PCOS）是一种常见的多因素和多基因内分泌系统疾病，影响多达20％的育龄妇女，病因仍然未知。卵泡液（FF）代表了富含代谢产物的卵泡正常发育的环境，激素和神经递质，但在某些情况下PCOS的组成可以是不同的。血管活性肠肽（VIP）是参与卵泡闭锁的内源性自主神经肽，颗粒细胞生理学和类固醇生成。
■进行ELISA测定以测量人卵泡液中的VIP和雌二醇水平，而AMH,FSH,LH,通过化学发光定量血浆中的雌二醇和孕酮。使用UHPLC/QTOF进行非靶向代谢组学分析。
■我们的ELISA和代谢组学结果显示：i）与对照组（n=10）相比，PCOS患者（n=9）的卵泡液中VIP浓度增加了约30％（132±28pg/ml对103±26pg/ml，p=0.03）在接受体外受精（IVF）的女性中，ii）VIP浓度与血清抗苗勒管激素（AMH）浓度之间呈线性正相关（p=0.05，r=0.45），iii）VIP与去甲肾上腺素代谢之间呈线性负相关。卵泡液中VIP和雌二醇（E2）浓度之间没有相关性。在卵泡液中发现VIP和去甲肾上腺素代谢物3,4-二羟基苯乙醇醛（DOPGAL）之间呈负相关。
■PCOS患者卵泡液中的VIP浓度增加，并且发现与去甲肾上腺素代谢相关，表明卵巢卵泡中交感神经反射可能失调。讨论了VIP作为去甲肾上腺素能调节剂在卵巢生理和PCOS病理生理中的作用。
■进行ELISA测定以测量人卵泡液中的VIP和雌二醇水平，而AMH,FSH,LH,通过化学发光定量血浆中的雌二醇和孕酮。使用UHPLC/QTOF进行非靶向代谢组学分析。
■我们的ELISA和代谢组学结果显示：i）与对照组（n=10）相比，PCOS患者（n=9）的卵泡液中VIP浓度增加了约30％（132±28pg/ml对103±26pg/ml，p=0.03）在接受体外受精（IVF）的女性中，ii）VIP浓度与血清抗苗勒管激素（AMH）浓度之间呈线性正相关（p=0.05，r=0.45），iii）VIP与去甲肾上腺素代谢之间呈线性负相关。卵泡液中VIP和雌二醇（E2）浓度之间没有相关性。在卵泡液中发现VIP和去甲肾上腺素代谢物3,4-二羟基苯乙醇醛（DOPGAL）之间呈负相关。
■PCOS患者卵泡液中的VIP浓度增加，并且发现与去甲肾上腺素代谢相关，表明卵巢卵泡中交感神经反射可能失调。讨论了VIP作为去甲肾上腺素能调节剂在卵巢生理和PCOS病理生理中的作用。
