UNASSIGNED: ELISA assays were performed to measure VIP and estradiol levels in human follicular fluids, while AMH, FSH, LH, estradiol and progesterone in the plasma were quantified by chemiluminescence. UHPLC/QTOF was used to perform the untargeted metabolomic analysis.
UNASSIGNED: Our ELISA and metabolomic results show: i) an increased concentration of VIP in follicular fluid of PCOS patients (n=9) of about 30% with respect to control group (n=10) (132 ± 28 pg/ml versus 103 ± 26 pg/ml, p=0,03) in women undergoing in vitro fertilization (IVF), ii) a linear positive correlation (p=0.05, r=0.45) between VIP concentration and serum Anti-Müllerian Hormone (AMH) concentration and iii) a linear negative correlation between VIP and noradrenaline metabolism. No correlation between VIP and estradiol (E2) concentration in follicular fluid was found. A negative correlation was found between VIP and noradrenaline metabolite 3,4-dihydroxyphenylglycolaldehyde (DOPGAL) in follicular fluids.
UNASSIGNED: VIP concentration in follicular fluids was increased in PCOS patients and a correlation was found with noradrenaline metabolism indicating a possible dysregulation of the sympathetic reflex in the ovarian follicles. The functional role of VIP as noradrenergic modulator in ovarian physiology and PCOS pathophysiology was discussed.
■进行ELISA测定以测量人卵泡液中的VIP和雌二醇水平,而AMH,FSH,LH,通过化学发光定量血浆中的雌二醇和孕酮。使用UHPLC/QTOF进行非靶向代谢组学分析。
■我们的ELISA和代谢组学结果显示:i)与对照组(n=10)相比,PCOS患者(n=9)的卵泡液中VIP浓度增加了约30%(132±28pg/ml对103±26pg/ml,p=0.03)在接受体外受精(IVF)的女性中,ii)VIP浓度与血清抗苗勒管激素(AMH)浓度之间呈线性正相关(p=0.05,r=0.45),iii)VIP与去甲肾上腺素代谢之间呈线性负相关。卵泡液中VIP和雌二醇(E2)浓度之间没有相关性。在卵泡液中发现VIP和去甲肾上腺素代谢物3,4-二羟基苯乙醇醛(DOPGAL)之间呈负相关。
■PCOS患者卵泡液中的VIP浓度增加,并且发现与去甲肾上腺素代谢相关,表明卵巢卵泡中交感神经反射可能失调。讨论了VIP作为去甲肾上腺素能调节剂在卵巢生理和PCOS病理生理中的作用。