Abstract：
Sensitive identification and effective inactivation of the virus are paramount for the early diagnosis and treatment of viral infections to prevent the risk of secondary transmission of viruses in the environment. Herein, we developed a novel two-step fluorescence immunoassay using antibody/streptavidin dual-labeled polystyrene nanobeads and biotin-labeled G-quadruplex/hemin DNAzymes with peroxidase-mimicking activity for sensitive quantitation and efficient inactivation of living Zika virus (ZIKV). The dual-labeled nanobeads can specifically bind ZIKV through E protein targeting and simultaneously accumulate DNAzymes, leading to the catalytic oxidation of Amplex Red indicators and generation of intensified aggregation-induced emission fluorescence signals, with a detection limit down to 66.3 PFU/mL and 100% accuracy. Furthermore, robust reactive oxygen species generated in situ by oxidized Amplex Red upon irradiation can completely kill the virus. This sensitive and efficient detection-inactivation integrated system will expand the viral diagnostic tools and reduce the risk of virus transmission in the environment.
摘要：
病毒的灵敏鉴定和有效灭活对于病毒感染的早期诊断和治疗以防止病毒在环境中二次传播的风险至关重要。在这里，我们使用抗体/链霉亲和素双重标记的聚苯乙烯纳米珠和生物素标记的具有过氧化物酶模拟活性的G-四链体/血红素DNA酶开发了一种新颖的两步荧光免疫测定法，用于灵敏定量和有效灭活活着的寨卡病毒（ZIKV）。双标记纳米珠可以通过E蛋白靶向与ZIKV特异性结合，同时积累DNA酶，导致AmplexRed指示剂的催化氧化，并产生增强的聚集诱导的发射荧光信号，检测限低至66.3PFU/mL，准确度为100%。此外，辐射后由氧化的AmplexRed原位产生的强大的活性氧可以完全杀死病毒。这种灵敏而高效的检测-灭活集成系统将扩展病毒诊断工具并降低病毒传播在环境中的风险。
