PDF(Pubmed)
Abstract：
Coccidiosis, which is caused by Eimeria species, results in huge economic losses to the poultry industry. Arbor Acres (AA) broilers and yellow-feathered broilers are the dominant broilers in northern and southern China, respectively. However, their susceptibility to coccidiosis has not been fully compared. In this study, the susceptibility of yellow-feathered broilers, AA broilers and Lohmann pink layers to E. tenella was evaluated based on mortality rate, relative body weight gain rate, intestinal lesion score, oocyst output, anticoccidial index (ACI), and cecum weight and length. The yellow-feathered broilers were shown to produce significantly fewer oocysts with higher intestinal lesion score compared to AA broilers, which had the highest growth rates and ACI scores. Subsequently, changes in the cecal microbiota of the 3 chicken lines before and after high-dose infection (1 × 104 oocysts) with E. tenella were determined by 16S rRNA sequencing. The results showed that composition of the microbiota changed dramatically after infection. The abundance of Firmicutes and Bacteroidetes in the infected chickens decreased, and Proteobacteria increased significantly among the different chicken lines. At the genus level, Escherichia increased significantly in all 3 groups of infected chickens, but Lactobacillus decreased to 0% in the infected yellow-feathered broilers. The results of the study indicate that the susceptibility to E. tenella varies among the 3 chicken lines, and that changes in intestinal microbiota by E. tenella-infection among the different chicken lines had a similar trend, but to different degrees. This study provides basic knowledge of the susceptibility in the 3 chicken lines, which can be helpful for the control and prevention of coccidiosis.
摘要：
球虫病,这是由艾美球虫引起的，给家禽业造成了巨大的经济损失。乔木英亩（AA）肉鸡和黄羽肉鸡是中国北方和南方的优势肉鸡，分别。然而,他们对球虫病的易感性尚未得到充分比较。在这项研究中,黄羽肉鸡的易感性，根据死亡率评估AA肉鸡和Lohmann粉红色的E.tenella层，相对体重增加率，肠道病变评分,卵囊输出，抗球虫指数（ACI），盲肠重量和长度。与AA肉鸡相比，黄羽肉鸡产生的卵囊明显减少，肠道病变评分更高，增长率和ACI得分最高。随后，通过16SrRNA测序确定了大剂量感染（1×104个卵囊）前后3种鸡系盲肠微生物群的变化。结果表明，感染后微生物群的组成发生了巨大变化。感染的鸡中Firmicutes和拟杆菌的丰度下降，和变形杆菌在不同鸡系中显著增加。在属一级，在所有3组感染的鸡中，大肠杆菌显著增加，但感染黄羽肉鸡的乳酸杆菌下降到0%。研究结果表明，3种鸡系对E.tenella的易感性各不相同，并且不同鸡系中由E.tenella感染引起的肠道微生物群的变化具有相似的趋势，但程度不同。本研究提供了3种鸡品系易感性的基本知识，有助于控制和预防球虫病。
