PDF(Pubmed)
Abstract：
BACKGROUND: Tooth discoloration is a common concern in antimicrobial photodynamic therapy (aPDT) using various photosensitizers (PS). Toluidine Blue (TB), Methylene Blue (MB), Phthalocyanine (Pc), and 2-mercaptopyridine-substituted zinc phthalocyanine (TM-ZnPc) are among those studied, but their relative impacts on tooth discoloration remain unclear.
OBJECTIVE: This study aimed to compare the effects of TB, MB, Pc, and TM-ZnPc in aPDT on tooth discoloration, utilizing a controlled experimental setup.
METHODS: The study comprised seventy-five single-rooted incisors with root canals. Following meticulous preparation, a standardized area on the crown surface was designated for examination, and precise measurements of the initial tooth colors were recorded. Samples were randomly divided into five groups: Negative control, MB, TM, Pc, and TM-ZnPc. Photoactivation was performed using LED light, and color measurements were taken at multiple time points up to 90 days. Data were converted to Lab* color values of the CIE Lab* color system (International Commission on Illumination, Vienna, Austria), and ΔE values were calculated. Statistical analysis was performed using Two-way ANOVA and Post-Hoc Tukey tests (p < 0.05).
RESULTS: At day 7 and 30, TM-ZnPc and Pc caused less discoloration compared to MB and TB. TM-ZnPc caused more tooth discoloration compared to Pc (p < 0.05). Compared to baseline, MB and TM-ZnPc caused more tooth discoloration at 30 days and TB caused more tooth discoloration at 90 days (p < 0.05). No significant difference was observed in terms of tooth discoloration at all periods evaluated after Pc application (p > 0.05). All photosensitizers tested in the study caused tooth coloration.
CONCLUSIONS: All PS induced clinically detectable tooth discoloration, with TB and MB causing more significant discoloration compared to Pc and TM-ZnPc at certain time points. TM-ZnPc and Pc demonstrated more stable coloration levels over time, suggesting their potential reliability in aPDT applications. This study highlights the importance of selecting appropriate PS to minimize tooth discoloration in aPDT, with Pc showing promise in this regard.
OBJECTIVE: This study aimed to compare the effects of TB, MB, Pc, and TM-ZnPc in aPDT on tooth discoloration, utilizing a controlled experimental setup.
METHODS: The study comprised seventy-five single-rooted incisors with root canals. Following meticulous preparation, a standardized area on the crown surface was designated for examination, and precise measurements of the initial tooth colors were recorded. Samples were randomly divided into five groups: Negative control, MB, TM, Pc, and TM-ZnPc. Photoactivation was performed using LED light, and color measurements were taken at multiple time points up to 90 days. Data were converted to Lab* color values of the CIE Lab* color system (International Commission on Illumination, Vienna, Austria), and ΔE values were calculated. Statistical analysis was performed using Two-way ANOVA and Post-Hoc Tukey tests (p < 0.05).
RESULTS: At day 7 and 30, TM-ZnPc and Pc caused less discoloration compared to MB and TB. TM-ZnPc caused more tooth discoloration compared to Pc (p < 0.05). Compared to baseline, MB and TM-ZnPc caused more tooth discoloration at 30 days and TB caused more tooth discoloration at 90 days (p < 0.05). No significant difference was observed in terms of tooth discoloration at all periods evaluated after Pc application (p > 0.05). All photosensitizers tested in the study caused tooth coloration.
CONCLUSIONS: All PS induced clinically detectable tooth discoloration, with TB and MB causing more significant discoloration compared to Pc and TM-ZnPc at certain time points. TM-ZnPc and Pc demonstrated more stable coloration levels over time, suggesting their potential reliability in aPDT applications. This study highlights the importance of selecting appropriate PS to minimize tooth discoloration in aPDT, with Pc showing promise in this regard.
摘要：
背景：牙齿变色是使用各种光敏剂（PS）的抗微生物光动力疗法（aPDT）中的常见问题。甲苯胺蓝(TB),亚甲蓝(MB),酞菁(Pc),研究了2-巯基吡啶取代的酞菁锌（TM-ZnPc），但它们对牙齿变色的相对影响尚不清楚。
目的：本研究旨在比较结核病的影响，MB,PC,和TM-ZnPc在aPDT中对牙齿变色的影响，利用受控的实验装置。
方法：该研究包括75个带有根管的单根门牙。经过精心准备,在表冠表面上指定了一个标准化区域进行检查，并记录初始牙齿颜色的精确测量。将样品随机分为5组:阴性对照,MB,TM,PC,和TM-ZnPc。使用LED灯进行光活化，在长达90天的多个时间点进行颜色测量。数据转换为CIELab*颜色系统的Lab*颜色值(国际照明委员会，维也纳,奥地利),并计算ΔE值。使用双向ANOVA和Post-HocTukey检验进行统计学分析(p<0.05)。
结果：在第7天和第30天，与MB和TB相比，TM-ZnPc和Pc引起的变色较少。与Pc相比,TM-ZnPc引起更多的牙齿变色(p<0.05)。与基线相比，MB和TM-ZnPc在30天时引起更多的牙齿变色,并且TB在90天时引起更多的牙齿变色(p<0.05)。在Pc施用后评价的所有时期,在牙齿变色方面没有观察到显著差异(p>0.05)。在研究中测试的所有光敏剂都导致牙齿着色。
结论：所有PS诱导临床可检测的牙齿变色，与Pc和TM-ZnPc相比,TB和MB在某些时间点引起更显著的变色。随着时间的推移，TM-ZnPc和Pc显示出更稳定的着色水平，表明它们在aPDT应用中的潜在可靠性。这项研究强调了在aPDT中选择合适的PS以最大程度地减少牙齿变色的重要性，PC在这方面表现出了希望。
目的：本研究旨在比较结核病的影响，MB,PC,和TM-ZnPc在aPDT中对牙齿变色的影响，利用受控的实验装置。
方法：该研究包括75个带有根管的单根门牙。经过精心准备,在表冠表面上指定了一个标准化区域进行检查，并记录初始牙齿颜色的精确测量。将样品随机分为5组:阴性对照,MB,TM,PC,和TM-ZnPc。使用LED灯进行光活化，在长达90天的多个时间点进行颜色测量。数据转换为CIELab*颜色系统的Lab*颜色值(国际照明委员会，维也纳,奥地利),并计算ΔE值。使用双向ANOVA和Post-HocTukey检验进行统计学分析(p<0.05)。
结果：在第7天和第30天，与MB和TB相比，TM-ZnPc和Pc引起的变色较少。与Pc相比,TM-ZnPc引起更多的牙齿变色(p<0.05)。与基线相比，MB和TM-ZnPc在30天时引起更多的牙齿变色,并且TB在90天时引起更多的牙齿变色(p<0.05)。在Pc施用后评价的所有时期,在牙齿变色方面没有观察到显著差异(p>0.05)。在研究中测试的所有光敏剂都导致牙齿着色。
结论：所有PS诱导临床可检测的牙齿变色，与Pc和TM-ZnPc相比,TB和MB在某些时间点引起更显著的变色。随着时间的推移，TM-ZnPc和Pc显示出更稳定的着色水平，表明它们在aPDT应用中的潜在可靠性。这项研究强调了在aPDT中选择合适的PS以最大程度地减少牙齿变色的重要性，PC在这方面表现出了希望。
