Abstract：
Healing of complex wounds requires dressings that must, at least, not hinder and should ideally promote the activity of key healing cells, in particular fibroblasts. This in vitro study assessed the effects of three wound-dressings (a pure Ca2+ alginate: Algostéril®, a Ca2+ alginate + carboxymethylcellulose: Biatain alginate® and a polyacrylate impregnated with lipido-colloid matrix: UrgoClean®) on dermal fibroblast activity. The results showed the pure calcium alginate to be non-cytotoxic, whereas the other wound-dressings showed moderate to strong cytotoxicity. The two alginates stimulated fibroblast migration and proliferation, whereas the polyacrylate altered migration and had no effect on proliferation. The pure Ca2+ alginate significantly increased the TGF-β-induced fibroblast activation, which is essential to healing. This activation was confirmed by a significant increase in Vascular endothelial growth factor (VEGF) secretion and a higher collagen production. The other dressings reduced these fibroblast activities. The pure Ca2+ alginate was also able to counteract the inhibitory effect of NK cell supernatants on fibroblast migration. These in vitro results demonstrate that tested wound-dressings are not equivalent for fibroblast activation. Only Algostéril was found to promote all the fibroblast activities tested, which could contribute to its healing efficacy demonstrated in the clinic.
摘要：
复杂伤口的愈合需要敷料，至少,不妨碍并应理想地促进关键愈合细胞的活动，特别是成纤维细胞。这项体外研究评估了三种伤口敷料（纯Ca2藻酸盐：Algostéril®，aCa2藻酸盐羧甲基纤维素：Biatain藻酸盐®和浸渍有脂质胶体基质的聚丙烯酸酯：UrgoClean®）对真皮成纤维细胞活性的影响。结果表明，纯海藻酸钙无细胞毒性，而其他伤口敷料显示中等至强烈的细胞毒性。两种藻酸盐刺激成纤维细胞迁移和增殖，而聚丙烯酸酯改变了迁移，对增殖没有影响。纯Ca2+藻酸盐显著增加TGF-β诱导的成纤维细胞活化,这对治愈至关重要。血管内皮生长因子(VEGF)分泌的显著增加和更高的胶原产生证实了这种激活。其他敷料降低了这些成纤维细胞活性。纯Ca2+藻酸盐也能够抵消NK细胞上清液对成纤维细胞迁移的抑制作用。这些体外结果证明,所测试的伤口敷料对于成纤维细胞活化是不等效的。只有Algostéril被发现促进所有测试的成纤维细胞活性，这可能有助于其在临床上证明的治愈功效。
