Abstract：
Nonylphenol (NP) is an organic pollutant and endocrine disruptor chemical that has harmful effects on the environment and living organisms. This study looked at whether kidney tissues subjected to increasing doses of nonylphenol generated alterations in histopathologic, pro-inflammatory, and autophagic markers. Fifty rats were divided into five groups of ten each: group I: healthy group, II: control (corn oil), group III: 25 μl/kg NP, group IV: 50 μl/kg NP, group V: 75 μl/kg NP. The kidney tissue samples were obtained for histopathological, immunohistochemical, and biochemical analyses. The histological deteriorations observed in all NP groups included tubular epithelial cell degeneration, inflammation areas, and hemorrhage. The immunohistochemical investigations showed that NP significantly elevated the autophagy markers (Beclin-1, LC3/2, p62), pro-inflammatory cytokines (TNF-α, IL-6), HIF-1α, and eNOS in group III, IV and V compared with group I and II. The biochemical analysis also revealed that pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) increased in correlation with the NP doses, but only IL-1β reached statistical significance in NP treated rats kidney tissue. The biochemical findings have been confirmed by the histological studies. The damage to renal tissue caused by NP exposure may worsen it by increasing inflammatory and autophagic markers.
摘要：
壬基酚（NP）是一种有机污染物和内分泌干扰物化学物质，对环境和生物体具有有害影响。这项研究观察了肾脏组织是否接受增加剂量的壬基酚在组织病理学上产生了改变，促炎,和自噬标记。将50只大鼠分为5组,每组10只:I组:健康组,II：对照（玉米油），III组：25μl/kgNP，IV组：50μl/kgNP，组V:75μl/kgNP。获得肾脏组织样本进行组织病理学检查，免疫组织化学，和生化分析。在所有NP组中观察到的组织学恶化包括肾小管上皮细胞变性，炎症区域，和出血。免疫组织化学研究表明，NP显着升高自噬标志物（Beclin-1，LC3/2，p62），促炎细胞因子(TNF-α，IL-6),HIF-1α,和eNOS在第三组，IV和V组与I和II组比较。生化分析还显示，促炎细胞因子(TNF-α，IL-1β,和IL-6）与NP剂量相关增加，但在NP处理的大鼠肾组织中只有IL-1β达到统计学意义。组织学研究证实了生化发现。NP暴露对肾组织的损害可能通过增加炎症和自噬标志物而使其恶化。
