PDF(Pubmed)
Abstract：
Azole resistance in Aspergillus fumigatus (ARAf) is becoming a worldwide health threat due to increasing occurrence in the environment. However, environmental surveillance programs are not commonly in place and are lacking in Belgium. Since no data on the occurrence of ARAf and the presence of hotspots for the selection of azole resistance is available in Belgium, a first study on the prevalence of ARAf in the environment was conducted. A total of 232 air and compost or soil samples were taken from two composting facilities, and from horticultural and agricultural crops. The azole susceptibility pattern was determined using the EUCAST method (E. Def. 9.4), and the cyp51A gene and its promotor region were sequenced in A. fumigatus isolates with phenotypic azole resistance. Six pan-azole-resistant A. fumigatus isolates were identified, originating from compost and horticultural crops. Four isolates carried the TR34/L98H mutation, and one isolate carried the TR46/Y121F/T289A mutation. However, we did not observe any ARAf isolates from agricultural crops. In conclusion, this study reported the first TR34/L98H and TR46/Y121F/T289A mutation isolated from a composting facility and horticulture in Belgium. The implementation of standardization in environmental surveillance of A. fumigatus on a European level would be beneficial in order to identify hotspots.
The ubiquitous fungus Aspergillus fumigatus can cause serious invasive diseases in humans. Due to the extensive use of environmental azoles, an increase of clinical infections with azole-resistant A. fumigatus is seen. This pilot study aimed to estimate the prevalence of azole-resistant A. fumigatus in environmental reservoirs in Belgium.
The ubiquitous fungus Aspergillus fumigatus can cause serious invasive diseases in humans. Due to the extensive use of environmental azoles, an increase of clinical infections with azole-resistant A. fumigatus is seen. This pilot study aimed to estimate the prevalence of azole-resistant A. fumigatus in environmental reservoirs in Belgium.
摘要：
由于在环境中的增加,烟曲霉(ARAf)中的唑抗性正成为全球健康威胁。然而,在比利时，环境监测计划并不普遍，也缺乏。由于在比利时没有关于ARAf的发生和选择唑类抗性的热点的数据，对环境中ARAf的患病率进行了首次研究。从两个堆肥设施中共抽取232份空气及堆肥或土壤样本,以及园艺和农作物。使用EUCAST方法确定唑类敏感性模式(E.Def.9.4），并对具有表型唑抗性的烟曲霉分离株中的cyp51A基因及其启动子区域进行了测序。鉴定出6株泛唑耐药的烟曲霉，源自堆肥和园艺作物。四个分离株携带TR34/L98H突变,一个分离株携带TR46/Y121F/T289A突变。然而,我们没有从农作物中观察到任何ARAf分离株。总之,这项研究报道了第一个TR34/L98H和TR46/Y121F/T289A突变从比利时的堆肥设施和园艺中分离。在欧洲水平上对烟曲霉的环境监测实施标准化将有利于确定热点。
普遍存在的真菌烟曲霉可引起人类严重的侵袭性疾病。由于环境唑的广泛使用，观察到耐唑的烟曲霉的临床感染增加。这项初步研究旨在评估比利时环境水库中耐唑的烟曲霉的患病率。
普遍存在的真菌烟曲霉可引起人类严重的侵袭性疾病。由于环境唑的广泛使用，观察到耐唑的烟曲霉的临床感染增加。这项初步研究旨在评估比利时环境水库中耐唑的烟曲霉的患病率。
