Abstract：
Fusarium proliferatum is the main pathogen that causes Panax notoginseng root rot. The shortcomings of strong volatility and poor water solubility of Illicium verum essential oil (EO) limit its utilization. In this study, we prepared traditional emulsion (BDT) and nanoemulsion (Bneo) of I. verum EO by ultrasonic method with Tween-80 and absolute ethanol as solvents. The chemical components of EO, BDT, and Bneo were identified by gas chromatography-mass spectrometry (GC-MS) and the antifungal activity and mechanism were compared. The results show that Bneo has good stability and its particle size is 34.86 nm. The contents of (-) -anethole and estragole in Bneo were significantly higher than those in BDT. The antifungal activity against F. proliferatum was 5.8-fold higher than BDT. In the presence of I. verum EO, the occurrence of P. notoginseng root rot was significantly reduced. By combining transcriptome and metabolomics analysis, I. verum EO was found to be involved in the mutual transformation of pentose and glucuronic acid, galactose metabolism, streptomycin biosynthesis, carbon metabolism, and other metabolic pathways of F. proliferatum, and it interfered with the normal growth of F. proliferatum to exert antifungal effects. This study provide a theoretical basis for expanding the practical application of Bneo.
摘要：
镰刀菌是引起三七根腐病的主要病原。八角精油(EO)挥发性强、水溶性差的缺点限制了其利用。在这项研究中,以Tween-80和无水乙醇为溶剂，通过超声法制备了I.verumEO的传统乳液（BDT）和纳米乳液（Bneo）。EO的化学成分，BDT,通过气相色谱-质谱（GC-MS）鉴定和Bneo，并比较了其抗真菌活性和机理。结果表明，Bneo具有良好的稳定性，粒径为34.86nm。Bneo中（-）-茴香脑和雌二醇的含量显着高于BDT。对增殖F.的抗真菌活性比BDT高5.8倍。在I.verumEO的存在下，三七根腐病的发生显著减少。通过结合转录组和代谢组学分析，发现I.verumEO参与戊糖和葡萄糖醛酸的相互转化，半乳糖代谢，链霉素生物合成，碳代谢，和其他代谢途径。并干扰增殖菌的正常生长以发挥抗真菌作用。本研究为拓展Bneo的实际应用提供了理论依据。
