Abstract：
Autophagy regulates the formation of primary cilia, which in turn affects autophagy. The relationship between autophagy and cilia is known to be bidirectional although the specific mechanisms involved have yet to be elucidated. In this study, we found for the first time that ATG8 protein localizes in the basal body of the dorsal kineties and the base of the ventral cirri in Euplotes amieti. ATG8 protein maintains the structural integrity of cilia and plays a role in the construction of the cortical ciliature and microtubule cytoskeleton associated with cilia. ATG8 gene interference leads to the degradation of IFT88, the transport protein in cilia, thus inhibiting the generation of cilia, and affecting the swing of cilia. This influences the swimming speed and cilia pattern, leading to death in Euplotes amieti.
摘要：
自噬调节初级纤毛的形成，这反过来又影响自噬。已知自噬与纤毛之间的关系是双向的，尽管涉及的具体机制尚未阐明。在这项研究中,我们首次发现ATG8蛋白定位于Euplopotesamieti的背侧运动的基体和腹侧cirri的基部。ATG8蛋白维持纤毛的结构完整性，并在与纤毛相关的皮质纤毛和微管细胞骨架的构建中起作用。ATG8基因干扰导致纤毛转运蛋白IFT88降解,从而抑制纤毛的产生，影响纤毛的摆动。这会影响游泳速度和纤毛模式，导致Euppotesamieti死亡。
