Abstract：
The abnormal uric acid (UA) level in urine can serve as warning signals of many diseases, such as gout and metabolic cardiovascular diseases. The current methods for detecting UA face limitations of instrument dependence and the requirement for non-invasiveness, making it challenging to fulfill the need for home-based application. In this study, we designed an aptasensor that combined UA-specific transcriptional regulation and a fluorescent RNA aptamer for convenient urinary UA testing. The concentration of UA can be translated into the intensity of fluorescent signals. The aptasensor showed higher sensitivity and more robust anti-interference performance. UA levels in the urine of different volunteers could be accurately tested using this method. In addition, a paper-based aptasensor for UA self-testing was manufactured, in which the urinary UA levels could be determined using a smartphone-based colorimetric approach. This work not only demonstrates a new approach for the design of disease-associated aptasensor, but also offers promising ideas for home-based detection of UA.
摘要：
尿液中的尿酸（UA）水平异常可以作为许多疾病的警告信号，如痛风和代谢性心血管疾病。当前检测UA的方法面临着仪器依赖性的局限性和对非侵入性的要求，这使得满足基于家庭的应用程序的需求变得具有挑战性。在这项研究中,我们设计了一种aptasensor，该aptasensor结合了UA特异性转录调控和荧光RNA适体，以方便尿液UA检测。UA的浓度可以转化为荧光信号的强度。该传感器具有更高的灵敏度和更强大的抗干扰性能。使用该方法可以准确地测试不同志愿者尿液中的UA水平。此外,制造了用于UA自检的纸质aptasensor，其中可以使用基于智能手机的比色法确定尿UA水平。这项工作不仅展示了一种设计疾病相关aptasensor的新方法，但也为基于家庭的UA检测提供了有希望的想法。
