PDF(Pubmed)
Abstract：
Flow cytometry has made a significant contribution to the study of several complex fundamental mechanisms in plant cytogenetics, becoming a useful analytical tool to understand several mechanisms and processes underlying plant growth, development, and function. In this study, the genome size, DNA ploidy level, and A-T/G-C ratio were measured for the first time for two genotypes of chia, Salvia hispanica, an herbaceous plant commonly used in phytotherapy and nutrition. This study also evaluated, for the first time by flow cytometry, the capacity to produce organic acids of tissues stained with LysoTracker Deep Red after elicitation with either yeast extract or cadmium chloride. Rosmarinic acid content differed between the two chia varieties treated with different elicitor concentrations, compared with non-elicited plant material. Elicited tissues of both varieties contained a higher content of rosmarinic acid compared with non-elicited cultures, and cadmium chloride at 500 μM was much better than that at 1000 μM, which led to plant death. For both genotypes, a dose-response was observed with yeast extract, as the higher the concentration of elicitor used, the higher rosmarinic acid content, resulting also in better results and a higher content of rosmarinic acid compared with cadmium chloride. This study demonstrates that flow cytometry may be used as a taxonomy tool, to distinguish among very close genotypses of a given species and, for the first time in plants, that this approach can also be put to profit for a characterization of the cytoplasmic acid phase and the concomitant production of secondary metabolites of interest in vitro, with or without elicitation. KEY POINTS: • Genome size, ploidy level, A-T/G-C ratio, and cytoplasm acid phase of S. hispanica • Cytometry study of cytoplasm acid phase of LysoTracker Deep Red-stained plant cells • Yeast extract or cadmium chloride elicited rosmarinic acid production of chia tissues.
摘要：
流式细胞术对植物细胞遗传学中几种复杂的基本机制的研究做出了重大贡献。成为一种有用的分析工具，以了解植物生长的多种机制和过程，发展,和功能。在这项研究中,基因组大小，DNA倍性水平，首次对两种基因型的chia进行了A-T/G-C比值测量，西班牙丹参,一种常用于植物治疗和营养的草本植物。这项研究还评估了，第一次通过流式细胞术，用酵母提取物或氯化镉激发后，用LysoTracker深红染色的组织产生有机酸的能力。用不同激发子浓度处理的两个正大品种之间的迷迭香酸含量不同，与未引起的植物材料相比。与未激发培养物相比，两个品种的激发组织中迷迭香酸的含量更高，500μM的氯化镉比1000μM的氯化镉好得多，导致植物死亡。对于这两种基因型，用酵母提取物观察到剂量反应，随着使用的激发子浓度越高，迷迭香酸含量越高，与氯化镉相比，结果也更好，迷迭香酸含量更高。这项研究表明，流式细胞术可以用作分类学工具，为了区分给定物种的非常接近的基因型，第一次在植物中，这种方法也可以用于表征细胞质酸相和伴随的体外次级代谢产物的产生，有或没有启发。关键点：•基因组大小，倍性水平，A-T/G-C比值,•LysoTracker深红色染色的植物细胞的细胞质酸相的细胞测定法研究•酵母提取物或氯化镉引起了迷迭香酸的产生。
