PDF(Pubmed)
Abstract：
Reliable laboratory diagnostic results are key for evaluating and improving children\'s health. To interpret these results, child-specific reference intervals (RIs), which account for constant biological changes and physiological development with sex and age, are required, as recommended by the Clinical and Laboratory Standards Institute (CLSI). This study presents age- and sex-specific reference intervals for complete blood count (CBC) parameters in children (<1-12 years old) in the Northern Region of Ghana. In this cross-sectional study, 600 healthy children from randomly sampled schools in Tamale (the Northern Region) were recruited and screened. Data from 388 eligible children were used to nonparametrically determine the reference intervals of CBC parameters at the 2.5th and 97.5th percentiles. The CBC reference intervals were compared for variations in sex and age groups using the Wilcoxon rank-sum test. There were no statistically significant differences in most CBC parameters by sex (RBC, Hb, HCT, MCH, RDW (CV/SD), WBC, LYM#, MON#(%) NEU#(%), EOS#(%), and BAS#(%); p > 0.05) and age group (RBC, MCV, RDW (CV/SD), WBC, LYM#, MON#(%) NEU#(%), EOS#(%), and BAS%; p > 0.05). However, there were observable differences between this locally established CBC reference interval and that used for children at Tamale Teaching Hospital (manufacturer\'s RIs). This study emphasises the importance of determining reference intervals representative of the local child population and incorporating them into the current reporting system of laboratories in the Northern Region to ensure the provision of effective and efficient healthcare services.
摘要：
可靠的实验室诊断结果是评估和改善儿童健康的关键。为了解释这些结果，儿童特异性参考间隔(RI)，这说明了随着性别和年龄的不断的生物变化和生理发育，是必需的,根据临床和实验室标准研究所（CLSI）的建议。这项研究提供了加纳北部地区儿童（<1-12岁）的全血细胞计数（CBC）参数的年龄和性别特定参考间隔。在这项横断面研究中，从塔马利（北部地区）随机抽样的学校中招募并筛选了600名健康儿童。来自388名合格儿童的数据被用于非参数化确定CBC参数在第2.5和97.5百分位数的参考间隔。使用Wilcoxon秩和检验比较了性别和年龄组的CBC参考间隔。大多数CBC参数按性别（RBC，Hb,HCT,MCH,RDW(CV/SD),WBC,LYM#,MON#(%)NEU#(%),EOS#(%),和BAS#(%)；p>0.05)和年龄组(RBC，MCV,RDW(CV/SD),WBC,LYM#,MON#(%)NEU#(%),EOS#(%),和BAS%；p>0.05)。然而,当地建立的CBC参考区间与Tamale教学医院(制造商RI)儿童使用的CBC参考区间之间存在可观察到的差异.这项研究强调了确定代表当地儿童人口的参考间隔并将其纳入北部地区实验室的当前报告系统的重要性，以确保提供有效和高效的医疗保健服务。
