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Abstract：
BACKGROUND: Intra-oral halitosis (IOH) is bad breath produced locally by the mouth in addition to systemic diseases and is one of the main causes of interpersonal communication and psychological disorders in modern society. However, current treatment modalities still only alleviate IOH and do not eradicate it. Therefore, based on the differential performance of oral microecology in IOH patients, we propose a microbiota transplantation treatment aimed at restoring oral microecological balance and analyze its feasibility by oral flora colonization test in Wistar rats.
OBJECTIVE: Saliva flora samples were collected from IOH patients and healthy subjects to analyze the feasibility of oral microbiota transplantation (OMT) for the treatment of IOH by the Wistar rat oral flora colonization test.
METHODS: Seven patients with IOH who visited the First Affiliated Hospital of Xinjiang Medical University from June 2017 to June 2022 with the main complaint of halitosis and three healthy subjects were randomly selected. A Halimeter portable breath detector was used to record breath values and collect saliva flora samples. Sixteen SPF-grade male Wistar rats were housed in the Animal Experiment Center of Xinjiang Medical University and randomly divided into an experimental group (Group E) and a control group (Group C) for the oral flora colonization test. Species composition and associated metabolic analysis of oral flora during the Wistar rat test using 16SrRNA sequencing technology and PICRUSt metabolic analysis. Also, the changes in the breath values of the rats were recorded during the test.
RESULTS: The proportion of Porphyromonas, Fusobacterium, Leptotrichia, and Peptostreptococcus was significantly higher in group E compared to group C after colonization of salivary flora of IOH patients (all P < 0.05), and the abundance with Gemella was zero before colonization, while no colonization was seen in group C after colonization compared to baseline. PICRUSt metabolic analysis also showed significantly enhanced IOH-related metabolic pathways after colonization in group E (all P < 0.05), as well as significantly higher breath values compared to baseline and group C (all P < 0.0001). After colonization by salivary flora from healthy subjects, group E rats showed a decrease in the abundance of associated odor-causing bacteria colonization, a reduction in associated metabolism, and a significant decrease in breath values. In contrast, group C also showed differential changes in flora structure and breath values compared to baseline after salivary flora colonization of IOH patients.
CONCLUSIONS: OMT for IOH is a promising green treatment option, but the influence of environmental factors and individual differences still cannot be ignored.
OBJECTIVE: Saliva flora samples were collected from IOH patients and healthy subjects to analyze the feasibility of oral microbiota transplantation (OMT) for the treatment of IOH by the Wistar rat oral flora colonization test.
METHODS: Seven patients with IOH who visited the First Affiliated Hospital of Xinjiang Medical University from June 2017 to June 2022 with the main complaint of halitosis and three healthy subjects were randomly selected. A Halimeter portable breath detector was used to record breath values and collect saliva flora samples. Sixteen SPF-grade male Wistar rats were housed in the Animal Experiment Center of Xinjiang Medical University and randomly divided into an experimental group (Group E) and a control group (Group C) for the oral flora colonization test. Species composition and associated metabolic analysis of oral flora during the Wistar rat test using 16SrRNA sequencing technology and PICRUSt metabolic analysis. Also, the changes in the breath values of the rats were recorded during the test.
RESULTS: The proportion of Porphyromonas, Fusobacterium, Leptotrichia, and Peptostreptococcus was significantly higher in group E compared to group C after colonization of salivary flora of IOH patients (all P < 0.05), and the abundance with Gemella was zero before colonization, while no colonization was seen in group C after colonization compared to baseline. PICRUSt metabolic analysis also showed significantly enhanced IOH-related metabolic pathways after colonization in group E (all P < 0.05), as well as significantly higher breath values compared to baseline and group C (all P < 0.0001). After colonization by salivary flora from healthy subjects, group E rats showed a decrease in the abundance of associated odor-causing bacteria colonization, a reduction in associated metabolism, and a significant decrease in breath values. In contrast, group C also showed differential changes in flora structure and breath values compared to baseline after salivary flora colonization of IOH patients.
CONCLUSIONS: OMT for IOH is a promising green treatment option, but the influence of environmental factors and individual differences still cannot be ignored.
摘要：
背景：口腔内口臭（IOH）是口腔局部产生的除全身性疾病外的口臭，是现代社会人际交往和心理障碍的主要原因之一。然而,目前的治疗方式仍然只能缓解IOH，不能根除它。因此,基于IOH患者口腔微生态的差异表现，我们提出了一种旨在恢复口腔微生态平衡的微生物移植治疗方法，并通过Wistar大鼠口腔菌群定植试验分析了其可行性。
目的：收集IOH患者和健康受试者的唾液菌群样本，通过Wistar大鼠口腔菌群定植试验，分析口腔菌群移植（OMT）治疗IOH的可行性。
方法：随机选择2017年6月至2022年6月在新疆医科大学第一附属医院就诊的7例以口臭为主诉的IOH患者和3例健康体检者。使用Halimeter便携式呼吸检测器来记录呼吸值并收集唾液菌群样品。将16只SPF级雄性Wistar大鼠圈养在新疆医科大学动物实验中心,随机分为实验组(E组)和对照组(C组)进行口腔菌群定植试验。使用16SrRNA测序技术和PICRUSt代谢分析在Wistar大鼠测试期间口腔菌群的物种组成和相关代谢分析。此外，在测试过程中记录大鼠呼吸值的变化。
结果：卟啉的比例，梭杆菌,Leptotrichia,在IOH患者唾液菌群定植后，E组肽链球菌明显高于C组（均P<0.05），在定殖之前，Gemella的丰度为零，与基线相比，C组未见定植。PICRUSt代谢分析也显示E组定植后IOH相关代谢途径显著增强(均P<0.05),以及显著高于基线和C组的呼吸值（所有P<0.0001）。在健康受试者的唾液菌群定植后，E组大鼠显示相关的引起气味的细菌定植的丰度减少，相关新陈代谢的减少，呼吸值显着下降。相比之下,在IOH患者唾液菌群定植后，与基线相比，C组还显示菌群结构和呼吸值的差异变化。
结论：用于IOH的OMT是一种有前途的绿色治疗选择，但环境因素和个体差异的影响仍然不容忽视。
目的：收集IOH患者和健康受试者的唾液菌群样本，通过Wistar大鼠口腔菌群定植试验，分析口腔菌群移植（OMT）治疗IOH的可行性。
方法：随机选择2017年6月至2022年6月在新疆医科大学第一附属医院就诊的7例以口臭为主诉的IOH患者和3例健康体检者。使用Halimeter便携式呼吸检测器来记录呼吸值并收集唾液菌群样品。将16只SPF级雄性Wistar大鼠圈养在新疆医科大学动物实验中心,随机分为实验组(E组)和对照组(C组)进行口腔菌群定植试验。使用16SrRNA测序技术和PICRUSt代谢分析在Wistar大鼠测试期间口腔菌群的物种组成和相关代谢分析。此外，在测试过程中记录大鼠呼吸值的变化。
结果：卟啉的比例，梭杆菌,Leptotrichia,在IOH患者唾液菌群定植后，E组肽链球菌明显高于C组（均P<0.05），在定殖之前，Gemella的丰度为零，与基线相比，C组未见定植。PICRUSt代谢分析也显示E组定植后IOH相关代谢途径显著增强(均P<0.05),以及显著高于基线和C组的呼吸值（所有P<0.0001）。在健康受试者的唾液菌群定植后，E组大鼠显示相关的引起气味的细菌定植的丰度减少，相关新陈代谢的减少，呼吸值显着下降。相比之下,在IOH患者唾液菌群定植后，与基线相比，C组还显示菌群结构和呼吸值的差异变化。
结论：用于IOH的OMT是一种有前途的绿色治疗选择，但环境因素和个体差异的影响仍然不容忽视。
