PDF(Pubmed)
Abstract：
The detrimental effects of smoking are multisystemic and its effects on the eye health are significant. Smoking is a strong risk factor for age-related nuclear cataract, age-related macular degeneration, glaucoma, delayed corneal epithelial healing and increased risk of cystoid macular edema in patients with intermediate uveitis among others. We aimed to characterize the aqueous humor (AH) proteome in chronic smokers to gain insight into its perturbations and to identify potential biomarkers for smoking-associated ocular pathologies. Compared to the control group, chronic smokers displayed 67 (37 upregulated, 30 downregulated) differentially expressed proteins (DEPs). Analysis of DEPs from the biological point of view revealed that they were proteins involved in complement activation, lymphocyte mediated immunity, innate immune response, cellular oxidant detoxification, bicarbonate transport and platelet degranulation. From the molecular function point of view, DEPs were involved in oxygen binding, oxygen carrier activity, hemoglobin binding, peptidase/endopeptidase/cysteine-type endopeptidase inhibitory activity. Several of the upregulated proteins were acute phase reactant proteins such as clusterin, alpha-2-HS-glycoprotein, fibrinogen, alpha-1-antitrypsin, C4b-binding protein and serum amyloid A-2. Further research should confirm if these proteins might serve as biomarkers or therapeutic target for smoking-associated ocular diseases.
摘要：
吸烟的有害影响是多系统的,并且其对眼睛健康的影响是显著的。吸烟是年龄相关性核性白内障的重要危险因素，年龄相关性黄斑变性,青光眼,中度葡萄膜炎患者的角膜上皮愈合延迟和黄斑囊样水肿风险增加。我们旨在表征慢性吸烟者的房水（AH）蛋白质组，以深入了解其扰动并确定与吸烟相关的眼部病变的潜在生物标志物。与对照组相比,慢性吸烟者显示67(37个上调，30下调）差异表达蛋白（DEP）。从生物学角度分析DEP，发现它们是参与补体激活的蛋白质，淋巴细胞介导的免疫，先天免疫反应，细胞氧化剂解毒，碳酸氢盐转运和血小板脱粒。从分子功能的角度来看，DEP参与氧结合，氧载体活性，血红蛋白结合，肽酶/内肽酶/半胱氨酸型内肽酶抑制活性。几种上调的蛋白质是急性期反应蛋白，如簇集蛋白，α-2-HS-糖蛋白，纤维蛋白原，α-1-抗胰蛋白酶,C4b结合蛋白和血清淀粉样蛋白A-2。进一步的研究应该确认这些蛋白质是否可以作为吸烟相关眼部疾病的生物标志物或治疗靶标。
