PDF(Pubmed)
Abstract：
Crude oil hydrocarbons are considered major environmental pollutants and pose a significant threat to the environment and humans due to having severe carcinogenic and mutagenic effects. Bioremediation is one of the practical and promising technology that can be applied to treat the hydrocarbon-polluted environment. In this present study, rhamnolipid biosurfactant (BS) produced by Pseudomonas aeruginosa PP4 and green synthesized iron nanoparticles (G-FeNPs) from Lawsonia inermis was used to evaluate the biodegradation efficiency (BE) of crude oil. The surface analysis of G-FeNPs was carried out by using FESEM and HRTEM to confirm the size and shape. Further, the average size of the G-FeNPs was observed around 10 nm by HRTEM analysis. The XRD and Raman spectra strongly confirm the presence of iron nanoparticles with their respective peaks. The BE (%) of mixed degradation system-V (PP4+BS+G-FeNPs) was obtained about 82%. FTIR spectrum confirms the presence of major functional constituents (C=O, -CH3, C-O, and OH) in the residual oil content. Overall, this study illustrates that integrated nano-based bioremediation could be an efficient approach for hydrocarbon-polluted environments. This study is the first attempt to evaluate the G-FeNPs with rhamnolipid biosurfactant on the biodegradation of crude oil.
摘要：
原油碳氢化合物被认为是主要的环境污染物，由于具有严重的致癌和诱变作用，对环境和人类构成重大威胁。生物修复技术是一种实用且有前途的技术，可用于处理碳氢化合物污染的环境。在本研究中,由铜绿假单胞菌PP4和绿色合成的铁纳米颗粒（G-FeNPs）产生的鼠李糖脂生物表面活性剂（BS）用于评估原油的生物降解效率（BE）。通过使用FESEM和HRTEM进行G-FeNPs的表面分析以确认尺寸和形状。Further,通过HRTEM分析观察到G-FeNPs的平均尺寸在10nm左右。XRD和拉曼光谱强烈证实了具有各自峰的铁纳米颗粒的存在。混合降解体系-V(PP4+BS+G-FeNPs)的BE(%)获得约82%。FTIR光谱证实了主要功能成分的存在（C=O，-CH3,C-O,和OH)中的残余油含量。总的来说,这项研究表明，综合纳米生物修复可能是碳氢化合物污染环境的有效方法。这项研究是首次尝试评估G-FeNPs与鼠李糖脂生物表面活性剂对原油生物降解的影响。
