Abstract：
OBJECTIVE: To determine the mydriatic effect of topical 10% phenylephrine with 10 mg/mL rocuronium bromide and compare this protocol with and without pretreatment with proparacaine.
METHODS: Ten client-owned pet adult eastern box turtles (Terrapene carolina carolina).
METHODS: All turtles were sedated with 8 mg/kg alfaxalone intramuscularly. One group of four turtles received four 20 μL drops of 10% phenylephrine and four 20 μL drops of rocuronium bromide in the right eye. Another group of four turtles received one standard drop of proparacaine followed by four 20 μL drops of 10% phenylephrine and four 20 μL drops of rocuronium bromide in the right eye. Two control group turtles received four 20 μL drops of saline in the right eye. The left eye was untreated in all turtles. Drops of the same type were separated by 2 min while drops of different types were separated by 5 min. Pupil size was recorded at 0, 15, 30, 60, 90, 120, 180, 240, and 360 min after administration of the final drop.
RESULTS: Treatment with 10% phenylephrine and rocuronium bromide resulted in pupil diameter changes from baseline that were statistically significant from zero at 60, 90, and 120 min in the non-proparacaine group and 90 min in the proparacaine group. The time to peak effect was 90 min in the proparacaine group and 75 min in the non-proparacaine group. Saline-treated pupils in the control group decreased in diameter over the study period. Overall, the treated eyes of the proparacaine group and non-proparacaine group were not different from each other, but both dilated more than the control group.
CONCLUSIONS: Rocuronium bromide and 10% phenylephrine can produce effective and safe mydriasis in eastern box turtles, but there was wide interindividual variation in effectiveness. Proparacaine did not improve the mydriatic effect.
METHODS: Ten client-owned pet adult eastern box turtles (Terrapene carolina carolina).
METHODS: All turtles were sedated with 8 mg/kg alfaxalone intramuscularly. One group of four turtles received four 20 μL drops of 10% phenylephrine and four 20 μL drops of rocuronium bromide in the right eye. Another group of four turtles received one standard drop of proparacaine followed by four 20 μL drops of 10% phenylephrine and four 20 μL drops of rocuronium bromide in the right eye. Two control group turtles received four 20 μL drops of saline in the right eye. The left eye was untreated in all turtles. Drops of the same type were separated by 2 min while drops of different types were separated by 5 min. Pupil size was recorded at 0, 15, 30, 60, 90, 120, 180, 240, and 360 min after administration of the final drop.
RESULTS: Treatment with 10% phenylephrine and rocuronium bromide resulted in pupil diameter changes from baseline that were statistically significant from zero at 60, 90, and 120 min in the non-proparacaine group and 90 min in the proparacaine group. The time to peak effect was 90 min in the proparacaine group and 75 min in the non-proparacaine group. Saline-treated pupils in the control group decreased in diameter over the study period. Overall, the treated eyes of the proparacaine group and non-proparacaine group were not different from each other, but both dilated more than the control group.
CONCLUSIONS: Rocuronium bromide and 10% phenylephrine can produce effective and safe mydriasis in eastern box turtles, but there was wide interindividual variation in effectiveness. Proparacaine did not improve the mydriatic effect.
摘要：
目的：为了确定10mg/mL罗库溴铵局部使用10％去氧肾上腺素的散瞳作用，并比较该方案与不使用丙对卡因预处理的情况。
方法：十只客户拥有的宠物成年东部箱龟（Terrapenecarolinacarolina）。
方法：所有海龟均肌内注射8mg/kg阿法沙酮镇静。一组四只海龟在右眼中接受了四滴20μL的10％去氧肾上腺素和四滴20μL的罗库溴铵。另一组四只海龟接受了一滴标准的丙美卡因，然后在右眼中接受了四滴20μL的10％去氧肾上腺素和四滴20μL的罗库溴铵。两个对照组海龟在右眼中接受4滴20μL盐水。所有海龟的左眼均未处理。相同类型的液滴分离2分钟,而不同类型的液滴分离5分钟。在施用最终液滴后0、15、30、60、90、120、180、240和360分钟记录瞳孔大小。
结果：用10%去氧肾上腺素和罗库溴铵治疗导致瞳孔直径相对于基线的变化，在60、90和120分钟时，非丙对卡因组和丙对卡因组90分钟时，差异有统计学意义。达到峰值的时间在丙美卡因组中为90分钟，在非丙美卡因组中为75分钟。在研究期间，对照组中盐水处理的瞳孔直径减小。总的来说,丙对卡因组和非丙对卡因组的治疗眼睛没有区别，但两者都比对照组扩张得更多。
结论：罗库溴铵和10%去氧肾上腺素可在东部箱龟中产生有效和安全的散瞳，但是有效性存在很大的个体差异。普罗卡因不能改善散瞳作用。
方法：十只客户拥有的宠物成年东部箱龟（Terrapenecarolinacarolina）。
方法：所有海龟均肌内注射8mg/kg阿法沙酮镇静。一组四只海龟在右眼中接受了四滴20μL的10％去氧肾上腺素和四滴20μL的罗库溴铵。另一组四只海龟接受了一滴标准的丙美卡因，然后在右眼中接受了四滴20μL的10％去氧肾上腺素和四滴20μL的罗库溴铵。两个对照组海龟在右眼中接受4滴20μL盐水。所有海龟的左眼均未处理。相同类型的液滴分离2分钟,而不同类型的液滴分离5分钟。在施用最终液滴后0、15、30、60、90、120、180、240和360分钟记录瞳孔大小。
结果：用10%去氧肾上腺素和罗库溴铵治疗导致瞳孔直径相对于基线的变化，在60、90和120分钟时，非丙对卡因组和丙对卡因组90分钟时，差异有统计学意义。达到峰值的时间在丙美卡因组中为90分钟，在非丙美卡因组中为75分钟。在研究期间，对照组中盐水处理的瞳孔直径减小。总的来说,丙对卡因组和非丙对卡因组的治疗眼睛没有区别，但两者都比对照组扩张得更多。
结论：罗库溴铵和10%去氧肾上腺素可在东部箱龟中产生有效和安全的散瞳，但是有效性存在很大的个体差异。普罗卡因不能改善散瞳作用。
