UNASSIGNED: Mature and immature C-mGCs were obtained from 38 women with polycystic ovary syndrome who participated in an in vitro fertilization program. The C-mGCs were then divided into two groups: fresh and vitrified. The expression levels of target genes were assessed using real-time quantitative polymerase chain reaction.
UNASSIGNED: After vitrification, GDF-9 expression was significantly decreased among both mature and immature C-mGCs, with 0.2- and 0.1-fold changes, respectively (p<0.01). Similarly, FSHR expression in the mature and immature groups was reduced by 0.1- and 0.02-fold, respectively, following vitrification (p<0.01). The expression levels of the other genes, including BMP-15, LHR, connexin 37, survivin, and caspase 3, remained similar across the examined groups (p>0.05).
UNASSIGNED: Vitrification may compromise oocyte maturation through reduced GDF-9 and FSHR expression in C-mGCs after warming.
■从参与体外受精计划的38名多囊卵巢综合征妇女中获得成熟和未成熟的C-mGC。然后将C-mGC分成两组:新鲜的和玻璃化的。使用实时定量聚合酶链反应评估靶基因的表达水平。
■玻璃化后,GDF-9表达在成熟和未成熟的C-mGCs中显著降低,随着0.2倍和0.1倍的变化,分别(p<0.01)。同样,FSHR在成熟和未成熟组中的表达减少了0.1倍和0.02倍,分别,玻璃化后(p<0.01)。其他基因的表达水平,包括BMP-15,LHR,连接蛋白37幸存者,和半胱天冬酶3,在各实验组中保持相似(p>0.05)。
■玻璃化可能通过加热后C-mGCs中GDF-9和FSHR表达减少而损害卵母细胞成熟。