Abstract：
UNASSIGNED: Ovarian tissue vitrification is widely utilized for fertility preservation in prepubertal and adolescent female patients with cancer. The current literature includes reports of successful pregnancy and live birth following autografting. However, the effects of the vitrification process on cumulus-mural granulosa cells (C-mGCs)-somatic cells in ovarian tissue crucial for oocyte maturation and early embryonic development-remain unclear. This study was conducted to explore the impact of vitrification on the cellular function of C-mGCs by quantifying the expression of growth differentiation factor 9 (GDF-9), bone morphogenetic protein 15 (BMP-15), follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), connexin 37, survivin, and caspase 3.
UNASSIGNED: Mature and immature C-mGCs were obtained from 38 women with polycystic ovary syndrome who participated in an in vitro fertilization program. The C-mGCs were then divided into two groups: fresh and vitrified. The expression levels of target genes were assessed using real-time quantitative polymerase chain reaction.
UNASSIGNED: After vitrification, GDF-9 expression was significantly decreased among both mature and immature C-mGCs, with 0.2- and 0.1-fold changes, respectively (p<0.01). Similarly, FSHR expression in the mature and immature groups was reduced by 0.1- and 0.02-fold, respectively, following vitrification (p<0.01). The expression levels of the other genes, including BMP-15, LHR, connexin 37, survivin, and caspase 3, remained similar across the examined groups (p>0.05).
UNASSIGNED: Vitrification may compromise oocyte maturation through reduced GDF-9 and FSHR expression in C-mGCs after warming.
UNASSIGNED: Mature and immature C-mGCs were obtained from 38 women with polycystic ovary syndrome who participated in an in vitro fertilization program. The C-mGCs were then divided into two groups: fresh and vitrified. The expression levels of target genes were assessed using real-time quantitative polymerase chain reaction.
UNASSIGNED: After vitrification, GDF-9 expression was significantly decreased among both mature and immature C-mGCs, with 0.2- and 0.1-fold changes, respectively (p<0.01). Similarly, FSHR expression in the mature and immature groups was reduced by 0.1- and 0.02-fold, respectively, following vitrification (p<0.01). The expression levels of the other genes, including BMP-15, LHR, connexin 37, survivin, and caspase 3, remained similar across the examined groups (p>0.05).
UNASSIGNED: Vitrification may compromise oocyte maturation through reduced GDF-9 and FSHR expression in C-mGCs after warming.
摘要：
■卵巢组织玻璃化被广泛用于青春期前和青春期女性癌症患者的生育力保存。目前的文献包括自体移植后成功怀孕和活产的报道。然而,玻璃化过程对卵巢组织中对卵母细胞成熟和早期胚胎发育至关重要的卵丘-壁颗粒细胞（C-mGCs）-体细胞的影响尚不清楚。本研究通过定量检测生长分化因子9(GDF-9)的表达,探讨玻璃化对C-mGCs细胞功能的影响。骨形态发生蛋白15（BMP-15），卵泡刺激素受体(FSHR),黄体生成素受体（LHR），连接蛋白37幸存者,和胱天蛋白酶3。
■从参与体外受精计划的38名多囊卵巢综合征妇女中获得成熟和未成熟的C-mGC。然后将C-mGC分成两组:新鲜的和玻璃化的。使用实时定量聚合酶链反应评估靶基因的表达水平。
■玻璃化后，GDF-9表达在成熟和未成熟的C-mGCs中显著降低,随着0.2倍和0.1倍的变化，分别(p<0.01)。同样，FSHR在成熟和未成熟组中的表达减少了0.1倍和0.02倍，分别,玻璃化后(p<0.01)。其他基因的表达水平，包括BMP-15，LHR，连接蛋白37幸存者,和半胱天冬酶3，在各实验组中保持相似(p>0.05)。
■玻璃化可能通过加热后C-mGCs中GDF-9和FSHR表达减少而损害卵母细胞成熟。
■从参与体外受精计划的38名多囊卵巢综合征妇女中获得成熟和未成熟的C-mGC。然后将C-mGC分成两组:新鲜的和玻璃化的。使用实时定量聚合酶链反应评估靶基因的表达水平。
■玻璃化后，GDF-9表达在成熟和未成熟的C-mGCs中显著降低,随着0.2倍和0.1倍的变化，分别(p<0.01)。同样，FSHR在成熟和未成熟组中的表达减少了0.1倍和0.02倍，分别,玻璃化后(p<0.01)。其他基因的表达水平，包括BMP-15，LHR，连接蛋白37幸存者,和半胱天冬酶3，在各实验组中保持相似(p>0.05)。
■玻璃化可能通过加热后C-mGCs中GDF-9和FSHR表达减少而损害卵母细胞成熟。
