Abstract：
Organosulfur compounds derived from plants of the Allium genus, such as propyl-propane-thiosulfinate (PTS) and propyl-propane-thiosulfonate (PTSO), have been proposed as an alternative in antibiotic resistance. The aim of this study was to compare the activity of these substances with other antibiotics against clinical isolates of carbapenem-resistant (CAR-R) and carbapenem-susceptible (CAR-S) Gram-negative bacteria. A total of 126 clinical isolates of CAR-R and 155 CAR-S bacteria were selected, including Enterobacterales, A. baumannii and P. aeruginosa. The antibiotic susceptibility of all isolates was assessed using the microdilution and Kirby-Bauer methods for PTS, PTSO, amoxicillin/clavulanate, piperacillin/tazobactam, cefotaxime, ceftazidime, cefepime, imipenem, ciprofloxacin, and amikacin. Both PTS and PTSO demonstrated in vitro bactericidal activity against CAR-R Enterobacteriaceae and A. baumannii, with no significant difference in activity compared to their response against CAR-S isolates. However, both compounds were less active against P. aeruginosa than against any of the other bacteria, regardless of their resistance to carbapenems. In all cases, the minimum inhibitory concentration values of PTSO were significantly lower than those of PTS. These findings offer valuable information about the potential antibacterial use of these substances, particularly against infections that currently have limited therapeutic options.
摘要：
来自葱属植物的有机硫化合物，如丙基-丙烷-硫代亚磺酸盐（PTS）和丙基-丙烷-硫代磺酸盐（PTSO），已被提议作为抗生素耐药性的替代品。这项研究的目的是比较这些物质与其他抗生素对碳青霉烯类耐药（CAR-R）和碳青霉烯类敏感（CAR-S）革兰氏阴性菌的临床分离株的活性。共筛选出126株CAR-R临床分离菌和155株CAR-S,包括肠杆菌,鲍曼不动杆菌和铜绿假单胞菌。使用微量稀释和Kirby-Bauer方法评估所有分离株的抗生素敏感性，PTSO,阿莫西林/克拉维酸，哌拉西林/他唑巴坦,头孢噻肟,头孢他啶,头孢吡肟,亚胺培南,环丙沙星,还有阿米卡星.PTS和PTSO均显示了对CAR-R肠杆菌科和鲍曼不动杆菌的体外杀菌活性，与它们对CAR-S分离株的反应相比，活性没有显着差异。然而,两种化合物对铜绿假单胞菌的活性均低于其他任何细菌，不管它们对碳青霉烯类的抗性。在所有情况下,PTSO的最低抑菌浓度值明显低于PTS。这些发现提供了有关这些物质的潜在抗菌用途的有价值的信息，特别是针对目前治疗选择有限的感染。
