Abstract：
BACKGROUND: Water sample culturing is the reference method to detect Legionella spp. in sanitary facilities. Until 2017, UNE-EN ISO 11731 only included the GVPC medium, which inhibits interfering microbiota but hinders the growth of Legionella spp. To improve its recovery, the new standard incorporates the BCYE medium into the working protocol.
METHODS: We inoculated 1306 sanitary water samples onto BCYE and GVPC according to an accredited internal procedure. We compared the number of cfu/L of Legionella spp. detected in both media.
RESULTS: The median in BCYE was 2000 cfu/L higher than in GVPC (P = .000). In the presence of high amounts of interfering microbiota, both media were similar; in the absence or low interfering microbiota BCYE was four times more sensitive than GVPC (P = .000).
CONCLUSIONS: Including BCYE in the analysis of sanitary water significantly improves the recovery of Legionella spp. in low contaminated samples.
METHODS: We inoculated 1306 sanitary water samples onto BCYE and GVPC according to an accredited internal procedure. We compared the number of cfu/L of Legionella spp. detected in both media.
RESULTS: The median in BCYE was 2000 cfu/L higher than in GVPC (P = .000). In the presence of high amounts of interfering microbiota, both media were similar; in the absence or low interfering microbiota BCYE was four times more sensitive than GVPC (P = .000).
CONCLUSIONS: Including BCYE in the analysis of sanitary water significantly improves the recovery of Legionella spp. in low contaminated samples.
摘要：
背景：水样培养是检测军团菌属的参考方法。在卫生设施中。直到2017年，UNE-ENISO11731仅包括GVPC介质，抑制干扰微生物群，但阻碍军团菌属的生长。为了改善其恢复，新标准将BCYE培养基纳入工作协议。
方法：我们根据认可的内部程序将1306个卫生水样接种到BCYE和GVPC上。我们比较了军团菌的cfu/L数。在两种媒体中检测到。
结果：BCYE的中位数比GVPC高2000cfu/L（P=.000）。在存在大量干扰微生物群的情况下，两种培养基相似;在缺乏或低干扰微生物群的情况下,BCYE的敏感性是GVPC的4倍(P=.000).
结论：在卫生用水分析中加入BCYE可显著提高军团菌的回收率。在低污染样品中。
方法：我们根据认可的内部程序将1306个卫生水样接种到BCYE和GVPC上。我们比较了军团菌的cfu/L数。在两种媒体中检测到。
结果：BCYE的中位数比GVPC高2000cfu/L（P=.000）。在存在大量干扰微生物群的情况下，两种培养基相似;在缺乏或低干扰微生物群的情况下,BCYE的敏感性是GVPC的4倍(P=.000).
结论：在卫生用水分析中加入BCYE可显著提高军团菌的回收率。在低污染样品中。
