Abstract：
Protein from tiger nut meal (TNP) performance high nutritional value. This study optimized the extraction parameters for TNP (DES-TNP) using deep eutectic solvent, with HBD: HBA = 5:1, Liquid: Solid = 11:1, and the moisture content was 15 %. A comprehensive comparison was conducted with the protein extracted using alkali-soluble acid precipitation (ASAE-TNP). DES-TNP demonstrated significantly higher purity (76.21 ± 2.59 %) than ASAE-TNP (67.48 ± 1.11 %). Density functional theory confirmed the successful synthesis of DES and its strong interaction with TNP. Moreover, DES-TNP and ASAE-TNP were different in structure (microscopic, secondary, and tertiary) and molecular weight distribution. The discrepancy contributed to the different functional properties, DES-TNP exhibiting better solubility, emulsification and foaming properties at pH13 compared to ASAE-TNP. For nutritional properties, DES-TNP and ASAE-TNP exhibited similar amino acid composition and digestibility, but the total amino acid content of DES-TNP was higher. This study presented a novel method for the extraction and comprehensive utilization of TNP.
摘要：
来自虎坚果粉的蛋白质（TNP）表现出很高的营养价值。本研究使用低共熔溶剂优化了TNP（DES-TNP）的萃取参数，HBD:HBA=5:1,液体:固体=11:1,水分含量为15%。与使用碱溶性酸沉淀(ASAE-TNP)提取的蛋白质进行综合比较。DES-TNP的纯度(76.21±2.59%)明显高于ASAE-TNP(67.48±1.11%)。密度泛函理论证实了DES的成功合成及其与TNP的强相互作用。此外，DES-TNP和ASAE-TNP在结构上不同(微观上，次要,和三级)和分子量分布。差异导致了不同的功能特性，DES-TNP具有更好的溶解性，与ASAE-TNP相比，pH13时的乳化和发泡性能。对于营养特性，DES-TNP和ASAE-TNP表现出相似的氨基酸组成和消化率，但DES-TNP的总氨基酸含量较高。本研究为TNP的提取和综合利用提供了一种新的方法。
