PDF(Pubmed)
Abstract：
Calcium binding protein, spermatid associated 1 (CABS1) is a protein most widely studied in spermatogenesis. However, mRNA for CABS1 has been found in numerous tissues, albeit with little information about the protein. Previously, we identified CABS1 mRNA and protein in human salivary glands and provided evidence that in humans CABS1 contains a heptapeptide near its carboxyl terminus that has anti-inflammatory activities. Moreover, levels of an immunoreactive form of CABS1 were elevated in psychological stress. To more fully characterize human CABS1 we developed additional polyclonal and monoclonal antibodies to different sections of the protein and used these antibodies to characterize CABS1 in an overexpression cell lysate, human salivary glands, saliva, serum and testes using western blot, immunohistochemistry and bioinformatics approaches exploiting the Gene Expression Omnibus (GEO) database. CABS1 appears to have multiple molecular weight forms, consistent with its recognition as a structurally disordered protein, a protein with structural plasticity. Interestingly, in human testes, its cellular distribution differs from that in rodents and pigs, and includes Leydig cells, primary spermatogonia, Sertoli cells and developing spermatocytes and spermatids, Geodata suggests that CABS1 is much more widely distributed than previously recognized, including in the urogenital, gastrointestinal and respiratory tracts, as well as in the nervous system, immune system and other tissues. Much remains to be learned about this intriguing protein.
摘要：
钙结合蛋白,精子细胞相关蛋白1（CABS1）是精子发生中研究最广泛的蛋白质。然而,已经在许多组织中发现了CABS1的mRNA，尽管关于蛋白质的信息很少。以前,我们在人类唾液腺中鉴定了CABS1mRNA和蛋白,并提供了在人类中CABS1在其羧基末端附近含有具有抗炎活性的七肽的证据.此外，在心理压力下，CABS1的免疫反应性形式的水平升高。为了更充分地表征人CABS1，我们开发了针对蛋白质不同部分的多克隆和单克隆抗体，并使用这些抗体来表征过表达细胞裂解物中的CABS1。人类唾液腺,唾液,血清和睾丸使用蛋白质印迹，免疫组织化学和生物信息学方法利用基因表达综合(GEO)数据库。CABS1似乎具有多种分子量形式，与它作为结构无序蛋白质的识别一致，具有结构可塑性的蛋白质。有趣的是,在人类睾丸中，它的细胞分布不同于啮齿动物和猪，包括睾丸间质细胞，原发性精原细胞，支持细胞和发育中的精母细胞和精子细胞，地理数据表明，CABS1的分布比以前认识到的要广泛得多，包括泌尿生殖系统,胃肠道和呼吸道，以及在神经系统中，免疫系统和其他组织。关于这种有趣的蛋白质还有很多需要了解。
