METHODS: We performed a single-centre retrospective cohort study including 1522 women undergoing 3130 PGT cycles at a referral centre for PGT. A logistic regression analysis was performed to predict the CLBR per ovarian stimulation in women undergoing PGT-M by polymerase chain reaction (PCR) or single-nucleotide polymorphism (SNP) array, and in women undergoing PGT-SR by SNP array, array comparative genomic hybridization (CGH) or next-generation sequencing (NGS).
RESULTS: The mean age of women was 32.6 years, with a mean AMH of 2.75 µg/L. Female age and AMH significantly affected the expected CLBR irrespective of the inheritance mode or PGT technology. An expected CLBR < 10% was reached above the age of 42 years and AMH ≤ 1.25 µg/L. We found no significant difference in outcome per ovarian stimulation between the different PGT technologies, i.e. PCR, SNP array, array CGH and NGS. Whereas per embryo transfer, we noticed a significantly higher probability of live birth when SNP array, array CGH and NGS were used as compared to PCR.
CONCLUSIONS: In a PGT-setting, couples with an unfavourable female age and AMH should be informed of the prognosis to allow other reproductive choices. The heatmap produced in this study can be used as a visual tool for PGT couples.
方法:我们进行了一项单中心回顾性队列研究,包括1522名在PGT转诊中心接受3130个PGT周期的妇女。通过聚合酶链反应(PCR)或单核苷酸多态性(SNP)阵列进行逻辑回归分析,以预测接受PGT-M的女性每次卵巢刺激的CLBR。在接受SNP阵列PGT-SR的女性中,阵列比较基因组杂交(CGH)或下一代测序(NGS)。
结果:女性平均年龄为32.6岁,平均AMH为2.75µg/L。无论遗传方式或PGT技术如何,女性年龄和AMH都会显着影响预期的CLBR。在42岁以上,AMH≤1.25µg/L的年龄达到预期的CLBR<10%。我们发现不同PGT技术之间每次卵巢刺激的结局没有显着差异,即PCR,SNP阵列,阵列CGH和NGS。而每次胚胎移植,我们注意到当SNP排列时,活产的概率明显更高,与PCR相比,使用阵列CGH和NGS。
结论:在PGT设置中,女性年龄不利且AMH的夫妇应被告知预后,以允许其他生殖选择.本研究中产生的热图可以用作PGT夫妇的可视化工具。