Abstract：
UNASSIGNED: Melanin is considered the main chromophore for laser hair removal. Due to a lack of laser-absorbing chromophores, removing non-pigmented hair with laser is quite problematic with unsatisfactory outcomes. This problem could be solved by delivering more melanin to the area around the hair follicle and enhancing that area as a target for light absorption. The insolubility of Sepia melanin as an exogenous dye, in most solvents, limits its bioavailability and thus its clinical use.
UNASSIGNED: In our study, to overcome the solubility problems and increase the bioavailability of melanin for biomedical and cosmetic applications, natural sepia melanin was loaded in different nano-delivery systems (spanlastics and transfersomes) to be delivered to the hair follicles. The different formulations of melanin were prepared and characterized. In vivo skin deposition and histopathological studies were conducted on albino mice.
UNASSIGNED: Transmission electron microscopy (TEM) showed the spherical shape of the prepared vesicles with an average particle size of 252 and 262 nm and zeta potential of -22.5 and -35 mV for melanin spanlastics and melanin transfersomes, respectively. Histopathological examination of hair follicles and pilosebaceous glands for the irradiated and non-irradiated albino mice skin was studied post the application of the prepared formulations topically and subcutaneously. Qualitative statistical analysis was conducted and melanin transfersomes and melanin spanlastics showed significant damage to pilosebaceous glands and hair follicles with a p-value of 0.031 and 0.009 respectively.
UNASSIGNED: Melanin nanovesicles as transfersomes and spanlastics could be considered a promising approach for the removal of non-pigmented hair.
UNASSIGNED: In our study, to overcome the solubility problems and increase the bioavailability of melanin for biomedical and cosmetic applications, natural sepia melanin was loaded in different nano-delivery systems (spanlastics and transfersomes) to be delivered to the hair follicles. The different formulations of melanin were prepared and characterized. In vivo skin deposition and histopathological studies were conducted on albino mice.
UNASSIGNED: Transmission electron microscopy (TEM) showed the spherical shape of the prepared vesicles with an average particle size of 252 and 262 nm and zeta potential of -22.5 and -35 mV for melanin spanlastics and melanin transfersomes, respectively. Histopathological examination of hair follicles and pilosebaceous glands for the irradiated and non-irradiated albino mice skin was studied post the application of the prepared formulations topically and subcutaneously. Qualitative statistical analysis was conducted and melanin transfersomes and melanin spanlastics showed significant damage to pilosebaceous glands and hair follicles with a p-value of 0.031 and 0.009 respectively.
UNASSIGNED: Melanin nanovesicles as transfersomes and spanlastics could be considered a promising approach for the removal of non-pigmented hair.
摘要：
目的黑色素被认为是激光脱毛的主要发色团。由于缺乏激光吸收发色团，用激光去除非色素毛发存在很大问题,结果不理想.这个问题可以通过将更多的黑色素输送到毛囊周围的区域并增强该区域作为光吸收的目标来解决。棕褐色黑色素作为外源染料的不溶性，在大多数溶剂中，限制了其生物利用度，从而限制了其临床应用。方法在我们的研究中,为了克服溶解度问题，提高黑色素的生物利用度，用于生物医学和化妆品应用，将天然棕褐色黑色素加载到不同的纳米递送系统(痉挛和传递体)中,以递送到毛囊。制备并表征黑色素的不同制剂。在白化病小鼠上进行体内皮肤沉积和组织病理学研究。结果透射电镜(TEM)显示所制备的囊泡呈球形,平均粒径分别为252和262nm,对于黑色素痉挛和黑色素传递体,zeta电位分别为-22.5和-35mV,分别。在局部和皮下施用制备的制剂后,研究了经照射和未经照射的白化病小鼠皮肤的毛囊和毛囊皮脂腺的组织病理学检查。进行了定性统计分析，黑色素传递体和黑色素插入体显示对毛囊和毛囊的显着损伤，p值分别为0.031和0.009。结论黑色素纳米囊泡作为传递体和插入体可以被认为是去除非色素毛发的有希望的方法。
