PDF(Pubmed)
Abstract：
Lactiplantibacillus plantarum M5 and Goji Berry extract were co-microencapsulated to maintain the activity of cells during gastrointestinal digestion, and the mechanism by which they could maintain high activity was investigated. The results showed that the microcapsules with 3% Goji Berry extract(A-GE-3) had the largest encapsulation efficiency(EE) of 92.41 ± 0.58%. SEM showed that the structure of A-GE-3 microcapsules were smoother and denser. Cell viability in A-GE-3 microcapsules remained at 7.17 log10 CFU/g after gastrointestinal digestion. Meanwhile, during the gastrointestinal digestion with 3% Goji Berry extract, cell membrane damage detected by fluorescent probes propidium iodide(PI) and 1.1-N-phenylnaphthylamine(NPN) was significantly reduced; the contents of arginine, glutamic acid and oleic acid in cell membrane were increased, which helped to maintain the dynamic balance of intracellular pH and regulated cell membrane fluidity in response to gastrointestinal environment. This study demonstrated the potential of Goji Berry extract as a probiotic protector in gastrointestinal digestion.
摘要：
植物乳杆菌M5和枸杞提取物共微囊化，以维持胃肠消化过程中细胞的活性，并研究了它们保持高活性的机制。结果表明，含3％枸杞提取物（A-GE-3）的微胶囊的最大包封率（EE）为92.41±0.58％。SEM显示,A-GE-3微胶囊结构更光滑、更致密。胃肠道消化后，A-GE-3微胶囊中的细胞活力保持在7.17log10CFU/g。同时,在使用3％枸杞提取物的胃肠道消化期间，荧光探针碘化丙啶(PI)和1.1-N-苯基萘胺(NPN)检测到的细胞膜损伤显著降低;细胞膜中的谷氨酸和油酸增加，这有助于维持细胞内pH的动态平衡，并调节细胞膜流动性以响应胃肠道环境。这项研究证明了枸杞提取物在胃肠道消化中作为益生菌保护剂的潜力。
