PDF(Pubmed)
Abstract：
UNASSIGNED: Aedes spp. are the most prolific mosquito vectors in the world. Found on every continent, they can effectively transmit various arboviruses, including the dengue virus which continues to cause outbreaks worldwide and is spreading into previously non-endemic areas. The lack of widely available dengue vaccines accentuates the importance of targeted vector control strategies to reduce the dengue burden. High-throughput tools to estimate human-mosquito contact and evaluate vector control interventions are lacking. We propose a novel serological tool that allows rapid screening of human cohorts for exposure to potentially infectious mosquitoes.
UNASSIGNED: We tested 563 serum samples from a longitudinal pediatric cohort study previously conducted in Cambodia. Children enrolled in the study were dengue-naive at baseline and were followed biannually for dengue incidence for two years. We used Western blotting and enzyme-linked immunosorbent assays to identify immunogenic Aedes aegypti salivary proteins and measure total anti-Ae. aegypti IgG.
UNASSIGNED: We found a correlation (rs=0.86) between IgG responses against AeD7L1 and AeD7L2 recombinant proteins and those to whole salivary gland homogenate. We observed seasonal fluctuations of AeD7L1+2 IgG responses and no cross-reactivity with Culex quinquefasciatus and Anopheles dirus mosquitoes. The baseline median AeD7L1+2 IgG responses for young children were higher in those who developed asymptomatic versus symptomatic dengue.
UNASSIGNED: The IgG response against AeD7L1+2 recombinant proteins is a highly sensitive and Aedes specific marker of human exposure to Aedes bites that can facilitate standardization of future serosurveys and epidemiological studies by its ability to provide a robust estimation of human-mosquito contact in a high-throughput fashion.
UNASSIGNED: We tested 563 serum samples from a longitudinal pediatric cohort study previously conducted in Cambodia. Children enrolled in the study were dengue-naive at baseline and were followed biannually for dengue incidence for two years. We used Western blotting and enzyme-linked immunosorbent assays to identify immunogenic Aedes aegypti salivary proteins and measure total anti-Ae. aegypti IgG.
UNASSIGNED: We found a correlation (rs=0.86) between IgG responses against AeD7L1 and AeD7L2 recombinant proteins and those to whole salivary gland homogenate. We observed seasonal fluctuations of AeD7L1+2 IgG responses and no cross-reactivity with Culex quinquefasciatus and Anopheles dirus mosquitoes. The baseline median AeD7L1+2 IgG responses for young children were higher in those who developed asymptomatic versus symptomatic dengue.
UNASSIGNED: The IgG response against AeD7L1+2 recombinant proteins is a highly sensitive and Aedes specific marker of human exposure to Aedes bites that can facilitate standardization of future serosurveys and epidemiological studies by its ability to provide a robust estimation of human-mosquito contact in a high-throughput fashion.
摘要：
■伊蚊。是世界上最多产的蚊媒。在每个大陆都有发现，它们可以有效地传播各种虫媒病毒，包括登革热病毒，该病毒继续在全球范围内爆发，并正在传播到以前的非流行地区。缺乏广泛可用的登革热疫苗突出了有针对性的媒介控制策略以减少登革热负担的重要性。缺乏评估人蚊接触和评估病媒控制干预措施的高通量工具。我们提出了一种新颖的血清学工具，可以快速筛查人类队列中是否暴露于潜在的传染性蚊子。
我们测试了先前在柬埔寨进行的一项纵向儿科队列研究的563份血清样本。纳入研究的儿童在基线时是未接受登革热治疗的，每两年随访一次登革热发病率。我们使用Western印迹和酶联免疫吸附测定来鉴定免疫原性埃及伊蚊唾液蛋白并测量总抗Ae。埃及伊蚊IgG。
■我们发现针对AeD7L1和AeD7L2重组蛋白的IgG应答与对整个唾液腺匀浆的应答之间存在相关性(rs=0.86)。我们观察到AeD7L12IgG反应的季节性波动，并且与库蚊和按蚊没有交叉反应。无症状登革热与有症状登革热相比，幼儿的基线中位AeD7L12IgG反应较高。
■针对AeD7L12重组蛋白的IgG反应是人类暴露于伊蚊叮咬的高度敏感性和伊蚊特异性标记，通过其能够提供可靠的能力，可以促进未来血清调查和流行病学研究的标准化。以高通量方式评估人蚊接触。
我们测试了先前在柬埔寨进行的一项纵向儿科队列研究的563份血清样本。纳入研究的儿童在基线时是未接受登革热治疗的，每两年随访一次登革热发病率。我们使用Western印迹和酶联免疫吸附测定来鉴定免疫原性埃及伊蚊唾液蛋白并测量总抗Ae。埃及伊蚊IgG。
■我们发现针对AeD7L1和AeD7L2重组蛋白的IgG应答与对整个唾液腺匀浆的应答之间存在相关性(rs=0.86)。我们观察到AeD7L12IgG反应的季节性波动，并且与库蚊和按蚊没有交叉反应。无症状登革热与有症状登革热相比，幼儿的基线中位AeD7L12IgG反应较高。
■针对AeD7L12重组蛋白的IgG反应是人类暴露于伊蚊叮咬的高度敏感性和伊蚊特异性标记，通过其能够提供可靠的能力，可以促进未来血清调查和流行病学研究的标准化。以高通量方式评估人蚊接触。
