Abstract：
QseC is a membrane sensor kinase that enables bacteria to perceive autoinducers -3, adrenaline, and norepinephrine to initiate downstream gene transcription. In this study, we found that the QseC protein of Glaesserella parasuis can serve as an effective antigen to activate the host\'s immune response. Therefore, we investigated the immunogenicity and host protective effect of this protein. ELISA and indirect immunofluorescence results showed that QseC protein can induce high titer levels of humoral immunity in mice and regularly generate specific serum antibodies. We used MTS reagents to detect lymphocyte proliferation levels and found that QseC protein can cause splenic lymphocyte proliferation with memory and specificity. Further immunological analysis of the spleen cell supernatant revealed significant upregulation of levels of IL-1β, IL-4 and IFN-γ in the QseC + adjuvant group. In the mouse challenge experiment, it was found that QseC + adjuvant can provide effective protection. The results of this study demonstrate that QseC protein provides effective protection in a mouse model and has the potential to serve as a candidate antigen for a novel subunit vaccine for further research.
摘要：
QseC是一种膜传感器激酶，使细菌能够感知自诱导因子-3，肾上腺素，和去甲肾上腺素启动下游基因转录。在这项研究中,我们发现副角松的QseC蛋白可以作为激活宿主免疫反应的有效抗原。因此,我们研究了该蛋白的免疫原性和宿主保护作用.ELISA和间接免疫荧光结果显示，QseC蛋白可诱导小鼠高滴度水平的体液免疫，并定期产生特异性血清抗体。我们使用MTS试剂检测淋巴细胞增殖水平，发现QseC蛋白可引起脾淋巴细胞增殖，具有记忆性和特异性。脾细胞上清液的进一步免疫分析显示IL-1β水平显著上调，QseC+佐剂组中的IL-4和IFN-γ。在老鼠挑战实验中，发现QseC+佐剂能提供有效的保护作用。这项研究的结果表明，QseC蛋白在小鼠模型中提供了有效的保护作用，并有可能作为新型亚单位疫苗的候选抗原进行进一步研究。
