Abstract：
Helicoverpa armigera, a ubiquitous polyphagous pest, poses a significant threat to global agriculture, causing substantial economic losses and demonstrating resistance to synthetic pesticides. This study investigates the potential of emamectin benzoate (EMB), an avermectin derivative, as an effective control agent against H. armigera. The larvae of the NBII-MP-NOC-01 strain of H. armigera were reared on an artificial diet. The impact of dietary EMB was examined on four midgut enzymes; alanine aminotransferase (ALT), aspartate aminotransferase (AST), acid phosphatase (ACP), and alkaline phosphatase (ALP). Results showed a dose-dependent and time-dependent reduction in ALT and AST activity, while an initial increase and subsequent decline in ACP and ALP activity at higher EMB concentrations. Computational modelling of enzyme structures and molecular docking studies revealed differential binding of EMB with the midgut enzymes. The strongest interaction was observed between EMB and ALT residues, contrasting with weakest interactions observed with AST. The study also showed that decreased activity of transaminases in H. armigera caused by EMB may be because of stability-activity trade-off, while in phosphatases reverse may be the case. This research provides crucial insights into the biochemical responses and the intricate insecticide-enzyme interactions in H. armigera caused by EMB exposure. This study lays the foundation for further research aimed at developing environmentally friendly approaches for managing H. armigera, addressing the challenges associated with conventional pesticides.
摘要：
棉铃虫，一种无处不在的多食性害虫，对全球农业构成重大威胁，造成巨大的经济损失和对合成农药的抗性。这项研究调查了甲氨基阿维菌素苯甲酸酯（EMB）的潜力，阿维菌素衍生物，作为对棉铃虫的有效控制剂。棉铃虫NBII-MP-NOC-01菌株的幼虫是用人工饮食饲养的。研究了饮食EMB对四种中肠酶的影响；丙氨酸转氨酶（ALT），天冬氨酸转氨酶（AST），酸性磷酸酶（ACP），碱性磷酸酶(ALP)。结果显示ALT和AST活性的剂量依赖性和时间依赖性降低，而在较高的EMB浓度下，ACP和ALP活性的初始增加和随后的下降。酶结构的计算模型和分子对接研究揭示了EMB与中肠酶的差异结合。在EMB和ALT残基之间观察到最强的相互作用，与AST观察到的最弱相互作用形成对比。该研究还表明，由EMB引起的棉铃虫转氨酶活性降低可能是由于稳定性-活性权衡，而在磷酸酶中可能是相反的情况。这项研究为EMB暴露引起的棉铃虫的生化反应和复杂的杀虫剂-酶相互作用提供了重要的见解。本研究为进一步研究奠定了基础，旨在开发环境友好的管理棉铃虫的方法，解决与常规农药相关的挑战。
