PDF(Pubmed)
Abstract：
BACKGROUND: Batai virus (BATV) is a zoonotic arbovirus of veterinary importance. A high seroprevalence in cows, sheep and goats and infection in different mosquito species has been observed in Central Europe. Therefore, we studied indigenous as well as exotic species of the genera Culex and Aedes for BATV vector competence at different fluctuating temperature profiles.
METHODS: Field caught Culex pipiens biotype pipiens, Culex torrentium, Aedes albopictus and Aedes japonicus japonicus from Germany and Aedes aegypti laboratory colony were infected with BATV strain 53.3 using artificial blood meals. Engorged mosquitoes were kept under four (Culex species) or three (Aedes species) fluctuating temperature profiles (18 ± 5 °C, 21 ± 5 °C, 24 ± 5 °C, 27 ± 5 °C) at a humidity of 70% and a dark/light rhythm of 12:12 for 14 days. Transmission was measured by testing the saliva obtained by forced salivation assay for viable BATV particles. Infection rates were analysed by testing whole mosquitoes for BATV RNA by quantitative reverse transcription PCR.
RESULTS: No transmission was detected for Ae. aegypti, Ae. albopictus or Ae. japonicus japonicus. Infection was observed for Cx. p. pipiens, but only in the three conditions with the highest temperatures (21 ± 5 °C, 24 ± 5 °C, 27 ± 5 °C). In Cx. torrentium infection was measured at all tested temperatures with higher infection rates compared with Cx. p. pipiens. Transmission was only detected for Cx. torrentium exclusively at the highest temperature of 27 ± 5 °C.
CONCLUSIONS: Within the tested mosquito species, only Cx. torrentium seems to be able to transmit BATV if the climatic conditions are feasible.
METHODS: Field caught Culex pipiens biotype pipiens, Culex torrentium, Aedes albopictus and Aedes japonicus japonicus from Germany and Aedes aegypti laboratory colony were infected with BATV strain 53.3 using artificial blood meals. Engorged mosquitoes were kept under four (Culex species) or three (Aedes species) fluctuating temperature profiles (18 ± 5 °C, 21 ± 5 °C, 24 ± 5 °C, 27 ± 5 °C) at a humidity of 70% and a dark/light rhythm of 12:12 for 14 days. Transmission was measured by testing the saliva obtained by forced salivation assay for viable BATV particles. Infection rates were analysed by testing whole mosquitoes for BATV RNA by quantitative reverse transcription PCR.
RESULTS: No transmission was detected for Ae. aegypti, Ae. albopictus or Ae. japonicus japonicus. Infection was observed for Cx. p. pipiens, but only in the three conditions with the highest temperatures (21 ± 5 °C, 24 ± 5 °C, 27 ± 5 °C). In Cx. torrentium infection was measured at all tested temperatures with higher infection rates compared with Cx. p. pipiens. Transmission was only detected for Cx. torrentium exclusively at the highest temperature of 27 ± 5 °C.
CONCLUSIONS: Within the tested mosquito species, only Cx. torrentium seems to be able to transmit BATV if the climatic conditions are feasible.
摘要：
背景：巴泰病毒（BATV）是一种具有兽医学重要性的人畜共患虫媒病毒。奶牛的高血清阳性率，在中欧观察到绵羊和山羊以及不同蚊子的感染。因此,我们研究了库蚊和伊蚊属的本地和外来物种在不同波动温度曲线下的BATV矢量能力。
方法：现场捕获淡色库蚊生物型，库蚊，来自德国的白纹伊蚊和日本伊蚊以及埃及伊蚊实验室菌落使用人工血粉感染BATV菌株53.3。驯服的蚊子保持在四种（库蚊物种）或三种（伊蚊物种）波动的温度曲线（18±5°C，21±5°C,24±5°C,27±5°C）在70％的湿度和12：12的暗/亮节奏下持续14天。通过测试通过强制唾液测定获得的唾液来测量传输,以检测活的BATV颗粒。通过定量逆转录PCR测试整个蚊子的BATVRNA来分析感染率。
结果：未检测到Ae的传播。埃及伊蚊,Ae.白纹或Ae。刺槐。对于Cx观察到感染。p.pipiens,但是仅在温度最高的三种条件下（21±5°C，24±5°C,27±5°C)。在CX。与Cx相比，在所有测试温度下都测量了Torrenum感染，感染率更高。p.pipiens。仅检测到Cx的传输。Torrenum仅在最高温度为27±5°C。
结论：在测试的蚊子种类中，只有CX。如果气候条件可行，Torrentium似乎可以传输BATV。
方法：现场捕获淡色库蚊生物型，库蚊，来自德国的白纹伊蚊和日本伊蚊以及埃及伊蚊实验室菌落使用人工血粉感染BATV菌株53.3。驯服的蚊子保持在四种（库蚊物种）或三种（伊蚊物种）波动的温度曲线（18±5°C，21±5°C,24±5°C,27±5°C）在70％的湿度和12：12的暗/亮节奏下持续14天。通过测试通过强制唾液测定获得的唾液来测量传输,以检测活的BATV颗粒。通过定量逆转录PCR测试整个蚊子的BATVRNA来分析感染率。
结果：未检测到Ae的传播。埃及伊蚊,Ae.白纹或Ae。刺槐。对于Cx观察到感染。p.pipiens,但是仅在温度最高的三种条件下（21±5°C，24±5°C,27±5°C)。在CX。与Cx相比，在所有测试温度下都测量了Torrenum感染，感染率更高。p.pipiens。仅检测到Cx的传输。Torrenum仅在最高温度为27±5°C。
结论：在测试的蚊子种类中，只有CX。如果气候条件可行，Torrentium似乎可以传输BATV。
