PDF(Pubmed)
Abstract：
BACKGROUND: Pulmonary air leaks (PALs) due to visceral pleura injury during surgery is frequently observed after pulmonary resections and the complication is difficult to avoid in thoracic surgery. The development of postoperative PALs is the most common cause of prolonged hospitalization. Previously, we reported that PALs sealants using autologous dermal fibroblast sheets (DFSs) harvested from temperature-responsive culture dishes successfully closed intraoperative PALs during lung resection.
OBJECTIVE: In this study, we investigated the fate of human DFSs xenogenetically transplanted onto lung surfaces to seal PALs of immunocompromised rat. Dual-color FISH analyses of human fibroblast was employed to detect transplantation human cells on the lung surface.
RESULTS: One month after transplantation, FISH analyses revealed that transplanted human fibroblasts still composed a sheet-structure, and histology also showed that beneath the sheet\'s angiogenesis migrating into the sheets was observed from the recipient tissues. FISH analyses revealed that even at 3 months after transplantation, the transplanted human fibroblasts still remained in the sheet. Dual-color FISH analyses of the transplanted human fibroblasts were sparsely present as a result of the cells reaching the end of their lifespan, the cells producing extracellular matrix, and remained inside the cell sheet and did not invade the lungs of the host.
CONCLUSIONS: DFS-transplanted human fibroblasts showed that they are retained within cell sheets and do not invade the lungs of the host.
OBJECTIVE: In this study, we investigated the fate of human DFSs xenogenetically transplanted onto lung surfaces to seal PALs of immunocompromised rat. Dual-color FISH analyses of human fibroblast was employed to detect transplantation human cells on the lung surface.
RESULTS: One month after transplantation, FISH analyses revealed that transplanted human fibroblasts still composed a sheet-structure, and histology also showed that beneath the sheet\'s angiogenesis migrating into the sheets was observed from the recipient tissues. FISH analyses revealed that even at 3 months after transplantation, the transplanted human fibroblasts still remained in the sheet. Dual-color FISH analyses of the transplanted human fibroblasts were sparsely present as a result of the cells reaching the end of their lifespan, the cells producing extracellular matrix, and remained inside the cell sheet and did not invade the lungs of the host.
CONCLUSIONS: DFS-transplanted human fibroblasts showed that they are retained within cell sheets and do not invade the lungs of the host.
摘要：
背景：肺切除术后经常观察到由于手术期间内脏胸膜损伤引起的肺漏气（PAL），并且在胸外科手术中很难避免并发症。术后PAL的发展是住院时间延长的最常见原因。以前,我们报道,使用自体真皮成纤维细胞薄片(DFSs)的PALs封闭剂在肺切除术期间成功封闭了术中PALs.
目的：在本研究中，我们调查了人类DFSs异种移植到肺表面以密封免疫受损大鼠的PAL的命运。采用人成纤维细胞的双色FISH分析来检测肺表面上的移植人细胞。
结果：移植后一个月，FISH分析显示，移植的人成纤维细胞仍然构成片状结构，和组织学也显示，从受体组织中观察到片材下方的血管生成迁移到片材中。FISH分析显示，即使在移植后3个月，移植的人成纤维细胞仍然保留在薄片中。移植的人成纤维细胞的双色FISH分析由于细胞达到其寿命的终点而稀疏存在，产生细胞外基质的细胞，并留在细胞片内，没有侵入宿主的肺。
结论：DFS移植的人成纤维细胞显示它们保留在细胞片内并且不侵入宿主的肺。
目的：在本研究中，我们调查了人类DFSs异种移植到肺表面以密封免疫受损大鼠的PAL的命运。采用人成纤维细胞的双色FISH分析来检测肺表面上的移植人细胞。
结果：移植后一个月，FISH分析显示，移植的人成纤维细胞仍然构成片状结构，和组织学也显示，从受体组织中观察到片材下方的血管生成迁移到片材中。FISH分析显示，即使在移植后3个月，移植的人成纤维细胞仍然保留在薄片中。移植的人成纤维细胞的双色FISH分析由于细胞达到其寿命的终点而稀疏存在，产生细胞外基质的细胞，并留在细胞片内，没有侵入宿主的肺。
结论：DFS移植的人成纤维细胞显示它们保留在细胞片内并且不侵入宿主的肺。
