Abstract:
Two Gram-stain-negative strains, designed SYSU M86414T and SYSU M84420, were isolated from marine sediment samples of the South China Sea (Sansha City, Hainan Province, PR China). These strains were aerobic and could grow at pH 6.0-8.0 (optimum, pH 7.0), 4-37 °C (optimum, 28 °C), and in the presence of 0-10 % NaCl (w/v; optimum 3 %). The predominant respiratory menaquinone of strains SYSU M86414T and SYSU M84420 was MK-6. The primary cellular polar lipid was phosphatidylethanolamine. The major cellular fatty acids (>10 %) in both strains were iso-C15 : 0, iso-C15 : 1 G, and iso-C17 : 0 3-OH. The DNA G+C content of strains SYSU M86414T and SYSU M84420 were both 42.10 mol%. Phylogenetic analyses based on 16S rRNA gene sequences and core genes indicated that these novel strains belonged to the genus Flagellimonas and strain SYSU M86414T showed the highest 16S rRNA gene sequence similarity to Flagellimonas marinaquae JCM 11811T (98.83 %), followed by Flagellimonas aurea BC31-1-A7T (98.62 %), while strain SYSU M84420 had highest 16S rRNA gene sequence similarity to F. marinaquae JCM 11811T (98.76 %) and F. aurea BC31-1-A7T (98.55 %). Based on the results of polyphasic analyses, strains SYSU M86414T and SYSU M84420 should be considered to represent a novel species of the genus Flagellimonas, for which the name Flagellimonas halotolerans sp. nov. is proposed. The type strain of the proposed novel isolate is SYSU M86414T (=GDMCC 1.3806T=KCTC 102040T).
摘要:
两个革兰氏染色阴性菌株,设计的SYSUM86414T和SYSUM84420,是从南海的海洋沉积物样品中分离出来的(三沙市,海南省,公关中国)。这些菌株是需氧的,可以在pH6.0-8.0下生长(最佳,pH7.0),4-37°C(最佳,28°C),并且在0-10%NaCl(w/v;最佳3%)的存在下。SYSUM86414T和SYSUM84420菌株的主要呼吸道甲萘醌是MK-6。主要的细胞极性脂质是磷脂酰乙醇胺。两种菌株中的主要细胞脂肪酸(>10%)是iso-C15:0,iso-C15:1G,和iso-C17:03-OH。菌株SYSUM86414T和SYSUM84420的DNAGC含量均为42.10mol%。基于16SrRNA基因序列和核心基因的系统发育分析表明,这些新菌株属于鞭毛虫属,菌株SYSUM86414T与鞭毛虫JCM11811T的16SrRNA基因序列相似性最高(98.83%),其次是紫菜BC31-1-A7T(98.62%),而SYSUM84420菌株与马氏F.marinaquaeJCM11811T(98.76%)和F.aureaBC31-1-A7T(98.55%)的16SrRNA基因序列相似性最高。根据多相分析的结果,菌株SYSUM86414T和SYSUM84420应被视为代表鞭毛虫属的一种新物种,为此,名字为弗拉格利莫纳斯盐藻属sp。11月。是提议的。提出的新型分离株的类型菌株是SYSUM86414T(=GDMCC1.386T=KCTC102040T)。