Abstract：
Plastics, omnipresent in the environment, have become a global concern due to their durability and limited biodegradability, especially in the form of microparticles and nanoparticles. Polystyrene (PS), a key plastic type, is susceptible to fragmentation and surface alterations induced by environmental factors or industrial processes. With widespread human exposure through pollution and diverse industrial applications, understanding the physiological impact of PS, particularly in nanoparticle form (PS-NPs), is crucial. This study focuses on the interaction of PS-NPs with model blood proteins, emphasising the formation of a protein corona, and explores the subsequent contact with platelet membrane mimetics using experimental and theoretical approaches. The investigation involves αIIbβ3-expressing cells and biomimetic membranes, enabling real-time and label-free nanoscale precision. By employing quartz-crystal microbalance with dissipation monitoring studies, the concentration-dependent cytotoxic effects of differently functionalised ~210 nm PS-NPs on HEK293 cells overexpressing αIIbβ3 are evaluated in detail. The study unveils insights into the molecular details of PS-NP interaction with supported lipid bilayers, demonstrating that a protein corona formed in the presence of exemplary blood proteins offers protection against membrane damage, mitigating PS-NP cytotoxicity.
摘要：
塑料,在环境中无所不在，由于其耐久性和有限的生物降解性，已成为全球关注的问题，特别是微粒和纳米颗粒的形式。聚苯乙烯(PS),一种关键的塑料类型，易受环境因素或工业过程引起的碎裂和表面变化的影响。随着污染和各种工业应用的广泛人类暴露，了解PS的生理影响，特别是纳米颗粒形式(PS-NP)，至关重要。本研究的重点是PS-NP与模型血液蛋白的相互作用，强调蛋白质电晕的形成，并使用实验和理论方法探讨了随后与血小板膜模拟物的接触。该研究涉及表达αIIbβ3的细胞和仿生膜，实现实时和无标签的纳米级精度。通过使用石英晶体微天平进行耗散监测研究，详细评估了不同功能化的~210nmPS-NP对过表达αIIbβ3的HEK293细胞的浓度依赖性细胞毒性作用。该研究揭示了PS-NP与支持的脂质双层相互作用的分子细节，证明在示例性血液蛋白质存在下形成的蛋白质冠可以防止膜损伤，减轻PS-NP细胞毒性。
