Abstract：
Correlative light-electron microscopy (CLEM) has evolved in the last decades, especially after significant developments in sample preparation, imaging acquisition, software, spatial resolution, and equipment, including confocal, live-cell, super-resolution, and electron microscopy (scanning, transmission, focused ion beam, and cryo-electron microscopy). However, the recent evolution of different laser-related techniques, such as mass spectrometry imaging (MSI) and laser capture microdissection, could further expand spatial imaging capabilities into high-resolution OMIC approaches such as proteomic, lipidomics, small molecule, and drug discovery. Here, we will describe a protocol to integrate the detection of rare viral reservoirs with imaging mass spectrometry.
摘要：
相关的光电子显微镜（CLEM）在过去的几十年中发展起来，特别是在样品制备方面取得重大进展之后，成像采集,软件,空间分辨率,和设备,包括共焦,活细胞，超分辨率,和电子显微镜(扫描，传输,聚焦离子束，和低温电子显微镜）。然而,不同激光相关技术的最新发展，如质谱成像(MSI)和激光捕获显微切割，可以进一步扩展空间成像能力到高分辨率的OMIC方法，如蛋白质组学，脂质组学，小分子,和药物发现。这里,我们将描述一个协议，以整合罕见的病毒库的检测与成像质谱。
