Abstract：
Colorectal cancer (CRC) has emerged as a prevalent malignancy worldwide, exhibiting the high morbidity and mortality rates. Resolvin D1 (RvD1) can exert anti-inflammation and anti-cancer effects on various diseases. This study is aimed to explore the role of RvD1 in CRC cells. HCT15 and SW480 cells were stimulated with IL-6 in our study. A series of assays such as CCK-8, colony formation, wound healing, Transwell, Western blotting, and immunofluorescence staining were designed and conducted to figure out the role of RvD1 in CRC cells. RvD1 suppressed IL-6-induced SW480 and HCT15 cell proliferation. In addition, RvD1 inhibited IL-6-induced SW480 and HCT15 cell migration, invasion, and EMT process. In mechanism, RvD1 inhibited the activation of IL-6/STAT3 signaling in SW480 and HCT15 cells. Angoline strengthened the inhibitive effect of RvD1 on cell malignancy. RvD1 inhibited cell growth, migration, invasion and EMT process by inactivating IL-6/STAT3 signaling in CRC.
摘要：
结直肠癌（CRC）已成为世界范围内普遍存在的恶性肿瘤。表现出很高的发病率和死亡率。消退素D1(RvD1)可对多种疾病发挥抗炎、抗癌作用。本研究旨在探讨RvD1在CRC细胞中的作用。在我们的研究中,用IL-6刺激HCT15和SW480细胞。一系列的测定，如CCK-8，集落形成，伤口愈合，Transwell,西方印迹,设计并进行免疫荧光染色，以了解RvD1在CRC细胞中的作用。RvD1抑制IL-6诱导的SW480和HCT15细胞增殖。此外,RvD1抑制IL-6诱导的SW480和HCT15细胞迁移,入侵,和EMT流程。在机制上,RvD1抑制SW480和HCT15细胞中IL-6/STAT3信号传导的激活。安哥拉碱增强了RvD1对细胞恶性肿瘤的抑制作用。RvD1抑制细胞生长，迁移,通过灭活IL-6/STAT3信号传导在CRC中的侵袭和EMT过程。
