Abstract：
The application of many hydrophilic and hydrophobic nutraceuticals is limited by their poor solubility, chemical stability, and/or bioaccessibility. In this study, a novel Pickering high internal phase double emulsion co-stabilized by modified pea protein isolate (PPI) and sodium alginate (SA) was developed for the co-encapsulation of model hydrophilic (riboflavin) and hydrophobic (β-carotene) nutraceuticals. Initially, the effect of emulsifier type in the external water phase on emulsion formation and stability was examined, including commercial PPI (C-PPI), C-PPI-SA complex, homogenized and ultrasonicated PPI (HU-PPI), and HU-PPI-SA complex. The encapsulation and protective effects of these double emulsions on hydrophilic riboflavin and hydrophobic β-carotene were then evaluated. The results demonstrated that the thermal and storage stabilities of the double emulsion formulated from HU-PPI-SA were high, which was attributed to the formation of a thick biopolymer coating around the oil droplets, as well as thickening of the aqueous phase. Encapsulation significantly improved the photostability of the two nutraceuticals. The double emulsion formulated from HU-PPI-SA significantly improved the in vitro bioaccessibility of β-carotene, which was mainly attributed to inhibition of its chemical degradation under simulated acidic gastric conditions. The novel delivery system may therefore be used for the development of functional foods containing multiple nutraceuticals.
摘要：
许多亲水性和疏水性营养品的应用受到溶解性差的限制，化学稳定性,和/或生物可及性。在这项研究中,开发了一种由改性豌豆蛋白分离物（PPI）和海藻酸钠（SA）共稳定的新型Pickering高内相双重乳液，用于共封装模型亲水性（核黄素）和疏水性（β-胡萝卜素）营养品。最初，研究了外水相中乳化剂类型对乳液形成和稳定性的影响，包括商业PPI(C-PPI)，C-PPI-SA复合物，均质化和超声处理PPI（HU-PPI），和HU-PPI-SA复合物。然后评估了这些双重乳液对亲水性核黄素和疏水性β-胡萝卜素的包封和保护作用。结果表明，由HU-PPI-SA配制的双重乳液的热稳定性和储存稳定性高，这归因于在油滴周围形成厚的生物聚合物涂层，以及水相的增稠。包封显著提高了两种营养品的光稳定性。由HU-PPI-SA配制的双重乳液显着提高了β-胡萝卜素的体外生物可及性，这主要归因于在模拟酸性胃条件下抑制其化学降解。因此,该新型递送系统可用于开发含有多种营养食品的功能性食品。
