PDF(Pubmed)
Abstract：
Cysteine-rich receptor-like kinases (CRKs) play many important roles during plant development, including defense responses under both biotic and abiotic stress, reactive oxygen species (ROS) homeostasis, callose deposition and programmed cell death (PCD). However, there are few studies on the involvement of the CRK family in male sterility due to heat stress in wheat (Triticum aestivum L.). In this study, a genome-wide characterization of the CRK family was performed to investigate the structural and functional attributes of the wheat CRKs in anther sterility caused by heat stress. A total of 95 CRK genes were unevenly distributed on 18 chromosomes, with the most genes distributed on chromosome 2B. Paralogous homologous genes with Ka/Ks ratios less than 1 may have undergone strong purifying selection during evolution and are more functionally conserved. The collinearity analysis results of CRK genes showed that wheat and Arabidopsis (A. thaliana), foxtail millet, Brachypodium distachyon (B. distachyon), and rice have three, 12, 15, and 11 pairs of orthologous genes, respectively. In addition, the results of the network interactions of genes and miRNAs showed that five miRNAs were in the hub of the interactions map, namely tae-miR9657b-5p, tae-miR9780, tae-miR9676-5p, tae-miR164, and tae-miR531. Furthermore, qRT-PCR validation of the six TaCRK genes showed that they play key roles in the development of the mononuclear stage anthers, as all six genes were expressed at highly significant levels in heat-stressed male sterile mononuclear stage anthers compared to normal anthers. We hypothesized that the TaCRK gene is significant in the process of high-temperature-induced sterility in wheat based on the combination of anther phenotypes, paraffin sections, and qRT-PCR data. These results improve our understanding of their relationship.
摘要：
富含半胱氨酸的受体样激酶(CRKs)在植物发育过程中发挥着重要作用,包括生物和非生物胁迫下的防御反应，活性氧（ROS）稳态，call体沉积和程序性细胞死亡（PCD）。然而,关于CRK家族参与小麦（TriticumaestivumL.）热胁迫导致的雄性不育的研究很少。在这项研究中,对CRK家族进行了全基因组表征，以研究由热胁迫引起的花药不育中小麦CRK的结构和功能属性。共有95个CRK基因在18条染色体上分布不均,大多数基因分布在2B号染色体上。Ka/Ks比率小于1的同源同源基因可能在进化过程中经历了强烈的纯化选择，并且在功能上更加保守。CRK基因共线性分析结果表明,小麦和拟南芥(A.thaliana),谷草,短臂远齿（B.远速粒子)，米饭有三个，12、15和11对直系同源基因，分别。此外,基因和miRNAs的网络相互作用结果表明，5个miRNAs位于相互作用图的中心，即tae-miR9657b-5p，tae-miR9780,tae-miR9676-5p,tae-miR164和tae-miR531.此外，6个TaCRK基因的qRT-PCR验证表明，它们在单核期花药的发育中起着关键作用，因为与正常花药相比，所有六个基因在热应激雄性不育单核期花药中均以极显著水平表达。根据花药表型的组合，我们假设TaCRK基因在高温诱导的小麦不育过程中具有重要意义，石蜡切片,和qRT-PCR数据。这些结果提高了我们对它们关系的理解。
