PDF(Pubmed)
Abstract：
Patients who suffer from myofascial orofacial pain could affect their quality of life deeply. The pathogenesis of pain is still unclear. Our objective was to assess Whether Voltage-gated calcium channel α2δ-1(Cavα2δ-1) is related to myofascial orofacial pain. Rats were divided into the masseter tendon ligation group and the sham group. Compared with the sham group, the mechanical pain threshold of the masseter tendon ligation group was reduced on the 4th, 7th, 10th and 14th day after operation(P < 0.05). On the 14th day after operation, Cavα2δ-1 mRNA expression levels in trigeminal ganglion (TG) and the trigeminal spinal subnucleus caudalis and C1-C2 spinal cervical dorsal horn (Vc/C2) of the masseter tendon ligation group were increased (PTG=0.021, PVc/C2=0.012). Rats were divided into three groups. On the 4th day after ligating the superficial tendon of the left masseter muscle of the rats, 10 ul Cavα2δ-1 antisense oligonucleotide, 10 ul Cavα2δ-1 mismatched oligonucleotides and 10 ul normal saline was separately injected into the left masseter muscle of rats in Cavα2δ-1 antisense oligonucleotide group, Cavα2δ-1 mismatched oligonucleotides group and normal saline control group twice a day for 4 days. The mechanical pain threshold of the Cavα2δ-1 antisense oligonucleotides group was higher than Cavα2δ-1 mismatched oligonucleotides group on the 7th and 10th day after operation (P < 0.01). After PC12 cells were treated with lipopolysaccharide, Cavα2δ-1 mRNA expression level increased (P < 0.001). Cavα2δ-1 may be involved in the occurrence and development in myofascial orofacial pain.
摘要：
患有肌筋膜口面部疼痛的患者可能会严重影响其生活质量。疼痛的发病机制尚不清楚。我们的目的是评估电压门控钙通道α2δ-1（Cavα2δ-1）是否与肌筋膜口面部疼痛有关。将大鼠分为咬肌腱结扎组和假手术组。与假手术组相比，咬肌腱结扎术组的机械性痛阈在4日降低，Seven,术后第10天和第14天(P<0.05)。手术后的第14天，咬肌腱结扎组三叉神经节(TG)、三叉神经尾核下和C1-C2脊髓颈背角(Vc/C2)中Cavα2δ-1mRNA表达水平升高(PTG=0.021,PVc/C2=0.012)。将大鼠分为三组。结扎大鼠左侧咬肌浅肌腱后第4天,10ulCavα2δ-1反义寡核苷酸，将10ulCavα2δ-1错配寡核苷酸和10ul生理盐水分别注射入Cavα2δ-1反义寡核苷酸组大鼠左侧咬肌，Cavα2δ-1错配寡核苷酸组和生理盐水对照组每天两次，共4天。Cavα2δ-1反义寡核苷酸组术后第7天和第10天机械痛阈值高于Cavα2δ-1错配寡核苷酸组(P<0.01)。PC12细胞用脂多糖处理后，Cavα2δ-1mRNA表达水平升高(P<0.001)。Cavα2δ-1可能参与肌筋膜口面部疼痛的发生和发展。
