PDF(Pubmed)
Abstract：
Intracytoplasmic sperm injection (ICSI) is clinically used to treat obstructive/nonobstructive azoospermia. This study compared the efficacy of ICSI with cauda epididymal and testicular sperm in Wistar (WI) and Brown-Norway (BN) rats. The transfer of ICSI oocytes with cryopreserved epididymal and testicular WI sperm resulted in offspring production of 26.2% and 3.7%-4.7%, respectively (P < 0.05). Treatments for artificial oocyte activation (AOA) and acrosome removal improved pronuclear formation in BN-ICSI oocytes; however, only AOA treatment was effective in producing offspring (3.7%-6.5%). In the case of ICSI with testicular sperm (TESE-ICSI), one offspring (0.6%) was derived from the BN-TESE-ICSI oocytes. The application of AOA or a hypo-osmotic sperm suspension did not improve the production of TESE-ICSI offspring. Thus, outbred WI rat offspring can be produced by using ICSI and less efficiently by using TESE-ICSI. Challenges in producing offspring by using ICSI/TESE-ICSI in inbred BN strain require further investigation.
摘要：
卵胞浆内单精子注射（ICSI）在临床上用于治疗阻塞性/非阻塞性无精子症。这项研究比较了ICSI与Wistar（WI）和Brown-Norway（BN）大鼠附睾和睾丸精子的疗效。用冷冻保存的附睾和睾丸WI精子转移ICSI卵母细胞导致后代产量分别为26.2％和3.7％-4.7％，分别为(P<0.05)。人工卵母细胞活化(AOA)和顶体去除治疗改善了BN-ICSI卵母细胞的原核形成；然而，只有AOA治疗有效产生后代（3.7％-6.5％）。在带有睾丸精子的ICSI（TESE-ICSI）的情况下，一个后代（0.6％）来自BN-TESE-ICSI卵母细胞。AOA或低渗精子悬浮液的应用并未改善TESE-ICSI后代的产生。因此，可以使用ICSI产生近交WI大鼠后代，而使用TESE-ICSI则效率较低。在近交BN菌株中使用ICSI/TESE-ICSI生产后代的挑战需要进一步研究。
