Abstract：
Dimethylsulfoniopropionate (DMSP) is one of the most abundant sulfur-containing organic compounds on the earth, which is an important carbon and sulfur source and plays an important role in the global sulfur cycle. Marine microorganisms are an important group involved in DMSP metabolism. The strain Cobetia sp. D5 was isolated from seawater samples in the Yellow Sea area of Qingdao during an algal bloom. There is still limited knowledge on the capacity of DMSP utilization of Cobetia bacteria. The study reports the whole genome sequence of Cobetia sp. D5 to understand its DMSP metabolism pathway. The genome of Cobetia sp. D5 consists of a circular chromosome with a length of 4,233,985 bp and the GC content is 62.56%. Genomic analysis showed that Cobetia sp. D5 contains a set of genes to transport and metabolize DMSP, which can cleave DMSP to produce dimethyl sulphide (DMS) and 3-Hydroxypropionyl-Coenzyme A (3-HP-CoA). DMS diffuses into the environment to enter the global sulfur cycle, whereas 3-HP-CoA is catabolized to acetyl CoA to enter central carbon metabolism. Thus, this study provides genetic insights into the DMSP metabolic processes of Cobetia sp. D5 during a marine algal bloom, and contributes to the understanding of the important role played by marine bacteria in the global sulfur cycle.
摘要：
二甲基磺丙酸(DMSP)是地球上最丰富的含硫有机化合物之一,是重要的碳和硫源，在全球硫循环中起着重要作用。海洋微生物是参与DMSP代谢的重要群体。菌株Cobetiasp.D5是在藻华期间从青岛黄海地区的海水样品中分离出来的。关于Cobetia细菌的DMSP利用能力的知识仍然有限。该研究报告了Cobetiasp的全基因组序列。D5明白其DMSP代谢通路。Cobetiasp.的基因组。D5由长度为4,233,985bp的环状染色体组成，GC含量为62.56％。基因组分析表明，Cobetiasp。D5包含一组转运和代谢DMSP的基因，其可以切割DMSP以产生二甲基硫化物(DMS)和3-羟基丙酰基-辅酶A(3-HP-CoA)。DMS扩散到环境中进入全球硫循环，而3-HP-CoA被分解代谢为乙酰辅酶A进入中心碳代谢。因此，这项研究为Cobetiasp.的DMSP代谢过程提供了遗传见解。D5在海洋藻华期间，并有助于理解海洋细菌在全球硫循环中的重要作用。
