PDF(Pubmed)
Abstract：
Implant therapy is a common treatment option in dentistry and orthopedics, but its application is often associated with an increased risk of microbial contamination of the implant surfaces that cause bone tissue impairment. This study aims to develop two silver-enriched platelet-rich plasma (PRP) multifunctional scaffolds active at the same time in preventing implant-associated infections and stimulating bone regeneration. Commercial silver lactate (L) and newly synthesized silver deoxycholate:β-Cyclodextrin (B), were studied in vitro. Initially, the antimicrobial activity of the two silver soluble forms and the PRP enriched with the two silver forms has been studied on microbial planktonic cells. At the same time, the biocompatibility of silver-enriched PRPs has been assessed by an MTT test on human primary osteoblasts (hOBs). Afterwards, an investigation was conducted to evaluate the activity of selected concentrations and forms of silver-enriched PRPs in inhibiting microbial biofilm formation and stimulating hOB differentiation. PRP-L (0.3 µg/mm2) and PRP-B (0.2 µg/mm2) counteract Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans planktonic cell growth and biofilm formation, preserving hOB viability without interfering with their differentiation capability. Overall, the results obtained suggest that L- and B-enriched PRPs represent a promising preventive strategy against biofilm-related implant infections and demonstrate a new silver formulation that, together with increasing fibrin binding protecting silver in truncated cone-shaped cyclic oligosaccharides, achieved comparable inhibitory results on prokaryotic cells at a lower concentration.
摘要：
种植疗法是牙科和骨科的常见治疗选择，但它的应用通常与植入物表面微生物污染的风险增加有关，导致骨组织受损。这项研究旨在开发两种富含银的富血小板血浆（PRP）多功能支架，同时具有预防植入物相关感染和刺激骨再生的活性。商业乳酸银（L）和新合成的脱氧胆酸银：β-环糊精（B），进行了体外研究。最初，已在微生物浮游细胞上研究了两种银可溶形式和富含两种银形式的PRP的抗菌活性。同时,已通过对人类原代成骨细胞（hOB）的MTT测试评估了富含银的PRP的生物相容性。之后,进行了一项研究，以评估选定浓度和形式的富含银的PRP在抑制微生物生物膜形成和刺激hOB分化方面的活性。PRP-L（0.3µg/mm2）和PRP-B（0.2µg/mm2）可抵消金黄色葡萄球菌，表皮葡萄球菌和白色念珠菌浮游细胞生长和生物膜形成，保持hOB活力而不干扰其分化能力。总的来说,获得的结果表明，富含L和B的PRP代表了一种有前途的预防生物膜相关植入物感染的策略，并证明了一种新的银配方，与增加的纤维蛋白结合一起保护截短的锥形环状寡糖中的银，在较低浓度的原核细胞上获得了可比的抑制结果。
