PDF(Pubmed)
Abstract：
Goose erysipelas is a serious problem in waterfowl breeding in Poland. However, knowledge of the characteristics of Erysipelothrix rhusiopathiae strains causing this disease is limited. In this study, the antimicrobial susceptibility and serotypes of four E. rhusiopathiae strains from domestic geese were determined, and their whole-genome sequences (WGSs) were analyzed to detect resistance genes, integrative and conjugative elements (ICEs), and prophage DNA. Sequence type and the presence of resistance genes and transposons were compared with 363 publicly available E. rhusiopathiae strains, as well as 13 strains of other Erysipelothrix species. Four strains tested represented serotypes 2 and 5 and the MLST groups ST 4, 32, 242, and 243. Their assembled circular genomes ranged from 1.8 to 1.9 kb with a GC content of 36-37%; a small plasmid was detected in strain 1023. Strains 1023 and 267 were multidrug-resistant. The resistance genes detected in the genome of strain 1023 were erm47, tetM, and lsaE-lnuB-ant(6)-Ia-spw cluster, while strain 267 contained the tetM and ermB genes. Mutations in the gyrA gene were detected in both strains. The tetM gene was embedded in a Tn916-like transposon, which in strain 1023, together with the other resistance genes, was located on a large integrative and conjugative-like element of 130 kb designated as ICEEr1023. A minor integrative element of 74 kb was identified in strain 1012 (ICEEr1012). This work contributes to knowledge about the characteristics of E. rhusiopathiae bacteria and, for the first time, reveals the occurrence of erm47 and ermB resistance genes in strains of this species. Phage infection appears to be responsible for the introduction of the ermB gene into the genome of strain 267, while ICEs most likely play a key role in the spread of the other resistance genes identified in E. rhusiopathiae.
摘要：
鹅丹毒是波兰水禽繁殖中的一个严重问题。然而,对引起这种疾病的红斑丹毒丝菌菌株的特征的了解有限。在这项研究中,测定了来自家鹅的4株大黄病大肠杆菌的抗菌药物敏感性和血清型，并分析其全基因组序列(WGSs)以检测抗性基因，整合和共轭元素(ICE)，和预言DNA。将序列类型以及抗性基因和转座子的存在与363个公开可用的E.rhusiopathiae菌株进行了比较，以及13种其他丹毒菌株。测试的四个菌株代表血清型2和5以及MLST组ST4、32、242和243。它们组装的环状基因组范围为1.8至1.9kb，GC含量为36-37％；在1023菌株中检测到一个小质粒。菌株1023和267具有多重耐药性。菌株1023基因组中检测到的抗性基因分别为erm47、tetM、和lsaE-lnuB-ant(6)-Ia-spw簇，而菌株267含有tetM和ermB基因。在两个菌株中均检测到gyrA基因的突变。tetM基因嵌入Tn916样转座子中,在菌株1023中，与其他抗性基因一起，位于130kb的大型整合和共轭样元件上，称为ICEEr1023。在菌株1012（ICEEr1012）中鉴定出74kb的次要整合元素。这项工作有助于了解胡氏大肠杆菌细菌的特性，第一次,揭示了该物种菌株中erm47和ermB抗性基因的发生。噬菌体感染似乎是将ermB基因引入菌株267基因组的原因，而ICE很可能在rhusiopathiae中鉴定的其他抗性基因的传播中起关键作用。
