PDF(Pubmed)
Abstract：
Almond hull, a substantial byproduct comprising more than half of almond fresh weight, has recently gained attention due to its functionality and sustainability benefits. Despite heightened interest, information regarding its toxicity remains limited. In order to assess its genotoxic potential, we conducted Good Laboratory Practice-compliant in vitro and in vivo studies following Organization for Economic Co-operation and Development (OECD) guidelines. No evidence of toxicity or mutagenicity was observed in a bacterial reverse mutation assay using five tester strains, evaluating almond hull at concentrations up to 5 mg/plate, with or without metabolic activation. Almond hull did not induce chromosome structural damage in a chromosome aberration assay using Chinese hamster ovary cells, nor did it cause any spermatogonial chromosomal aberration in tested male BALB/c mice. To evaluate its ability to induce DNA damage in rodents, a combined micronucleus assay was conducted in KM mice of both sexes. Almond hull was administered at doses of 1250, 2500, and 5000 mg/kg/day via gavage once daily for 2 days. No adverse effects of almond hull were observed in the micronucleus assay. Our results indicate no evidence of the genotoxic potential of almond hull administered up to the maximum concentrations of 5 g/kg, as recommended by OECD guidelines.
摘要：
杏仁船体,占杏仁鲜重一半以上的大量副产品，由于其功能和可持续性优势，最近获得了关注。尽管兴趣提高了，关于其毒性的信息仍然有限。为了评估其基因毒性潜力，我们遵循经济合作与发展组织(OECD)指南进行了符合良好实验室规范的体外和体内研究.在使用五种测试菌株的细菌回复突变试验中未观察到毒性或诱变性的证据，评估浓度高达5毫克/板的杏仁壳，有或没有代谢激活。在使用中国仓鼠卵巢细胞的染色体畸变试验中，杏仁壳没有诱导染色体结构损伤，在测试的雄性BALB/c小鼠中也没有引起任何精原染色体畸变。为了评估其在啮齿动物中诱导DNA损伤的能力，在两种性别的KM小鼠中进行了联合微核测定。杏仁壳以1250、2500和5000mg/kg/天的剂量通过管饲法每天施用一次,持续2天。在微核试验中未观察到杏仁壳的不良反应。我们的结果表明，没有证据表明杏仁壳的遗传毒性潜力，最高浓度为5g/kg，按照经合组织准则的建议。
