PDF(Pubmed)
Abstract：
With increasingly used assisted reproductive technology (ART), the acquisition of high-quality oocytes and early embryos has become the focus of much attention. Studies in mice have found that the transition of chromatin conformation from non-surrounded nucleolus (NSN) to surrounded nucleolus (SN) is essential for oocyte maturation and early embryo development, and similar chromatin transition also exists in human oocytes. In this study, we collected human NSN and SN oocytes and investigated their transcriptome. The analysis of differentially expressed genes showed that epigenetic functions, cyclin-dependent kinases and transposable elements may play important roles in chromatin transition during human oocyte maturation. Our findings provide new insights into the molecular mechanism of NSN-to-SN transition of human oocyte and obtained new clues for improvement of oocyte in vitro maturation technique.
摘要：
随着辅助生殖技术（ART）的应用日益广泛，获得高质量的卵母细胞和早期胚胎已成为备受关注的焦点。在小鼠中的研究发现，染色质构象从非包围核仁（NSN）到包围核仁（SN）的转变对于卵母细胞成熟和早期胚胎发育至关重要。人类卵母细胞中也存在类似的染色质转变。在这项研究中,我们收集了人类NSN和SN卵母细胞，并研究了它们的转录组。差异表达基因的分析表明,表观遗传功能,细胞周期蛋白依赖性激酶和转座因子可能在人类卵母细胞成熟过程中的染色质转变中起重要作用。我们的发现为人类卵母细胞NSN到SN转换的分子机制提供了新的见解，并为改进卵母细胞体外成熟技术提供了新的线索。
