PDF(Pubmed)
Abstract：
The majority of pigeon paramyxovirus type 1 (PPMV-1) strains are generally non-pathogenic to chickens; however, they can induce severe illness and high mortality rates in pigeons, leading to substantial economic repercussions. The genomes of 11 PPMV-1 isolates from deceased pigeons on meat pigeon farms during passive monitoring from 2009 to 2012 were sequenced and analyzed using polymerase chain reaction and phylogenetic analysis. The complete genome lengths of 11 isolates were approximately 15,192 nucleotides, displaying a consistent gene order of 3\'-NP-P-M-F-HN-L-5\'. ALL isolates exhibited the characteristic motif of 112RRQKRF117 at the fusion protein cleavage site, which is characteristic of velogenic Newcastle disease virus. Moreover, multiple mutations have been identified within the functional domains of the F and HN proteins, encompassing the fusion peptide, heptad repeat region, transmembrane domains, and neutralizing epitopes. Phylogenetic analysis based on sequences of the F gene unveiled that all isolates clustered within genotype VI in class II. Further classification identified at least two distinct sub-genotypes, with seven isolates classified as sub-genotype VI.2.1.1.2.2, whereas the others were classified as sub-genotype VI.2.1.1.2.1. This study suggests that both sub-genotypes were implicated in severe disease manifestation among meat pigeons, with sub-genotype VI.2.1.1.2.2 displaying an increasing prevalence among Shanghai\'s meat pigeon population since 2011. These results emphasize the value of developing pigeon-specific vaccines and molecular diagnostic tools for monitoring and proactively managing potential PPMV-1 outbreaks.
摘要：
大多数鸽子副粘病毒1型（PPMV-1）菌株通常对鸡无致病性；然而，它们可以在鸽子中诱发严重的疾病和高死亡率，导致重大的经济影响。对2009年至2012年期间在肉鸽养殖场被动监测过程中从死亡鸽子中分离出的11株PPMV-1的基因组进行了测序，并使用聚合酶链反应和系统发育分析进行了分析。11个分离株的完整基因组长度约为15,192个核苷酸，显示3'-NP-P-M-F-HN-L-5'的一致基因顺序。所有分离物在融合蛋白切割位点表现出112RRQKRF117的特征性基序，这是新城疫病毒的特征。此外，已在F和HN蛋白的功能结构域中鉴定出多种突变，包括融合肽，七肽重复区，跨膜结构域，和中和表位。基于F基因序列的系统发育分析揭示了所有分离株在II类中聚集在基因型VI内。进一步分类确定了至少两种不同的亚基因型，有七个分离株被分类为子基因型VI.1.1.1.2.2，而其他分离株被分类为子基因型VI.1.1.1.1。这项研究表明，这两种亚基因型都与肉鸽的严重疾病表现有关，子基因型VI.2.1.1.2.2显示，自2011年以来，上海肉鸽种群的患病率有所增加。这些结果强调了开发鸽子特异性疫苗和分子诊断工具对监测和主动管理潜在的PPMV-1爆发的价值。
