Abstract：
Cell culture meat is based on the scaled-up expansion of seed cells. The biological differences between seed cells from large yellow croakers in the two-dimensional (2D) and three-dimensional (3D) culture systems have not been explored. Here, satellite cells (SCs) from large yellow croakers (Larimichthys crocea) were grown on cell climbing slices, hydrogels, and microcarriers for five days to analyze the biological differences of SCs on different cell scaffolds. The results exhibited that SCs had different cell morphologies in 2D and 3D cultures. Cell adhesion receptors (Itgb1andsdc4) and adhesion spot markervclof the 3D cultures were markedly expressed. Furthermore, myogenic decision markers (Pax7andmyod) were significantly enhanced. However, the expression of myogenic differentiation marker (desmin) was significantly increased in the microcarrier group. Combined with the transcriptome data, this suggests that cell adhesion of SCs in 3D culture was related to the integrin signaling pathway. In contrast, the slight spontaneous differentiation of SCs on microcarriers was associated with rapid cell proliferation. This study is the first to report the biological differences between SCs in 2D and 3D cultures, providing new perspectives for the rapid expansion of cell culture meat-seeded cells and the development of customized scaffolds.
摘要：
细胞培养肉是基于种子细胞的放大扩增。尚未探索二维（2D）和三维（3D）培养系统中大黄鱼的种子细胞之间的生物学差异。这里,来自大黄鱼（Larimichthyscrocea）的卫星细胞（SC）在细胞攀爬切片上生长，水凝胶,5天微载体,分析SCs在不同细胞支架上的生物学差异。结果表明，SC在2D和3D培养中具有不同的细胞形态。3D培养物的细胞粘附受体（Itgb1和sdc4）和粘附点标记明显表达。此外，肌源性决定标记（Pax7andmyod）显着增强。然而,微载体组中成肌分化标志物(desmin)的表达显著增加。结合转录组数据，这表明3D培养中SCs的细胞粘附与整合素信号通路有关。相比之下,SCs在微载体上的轻微自发分化与细胞快速增殖有关。这项研究首次报道了2D和3D培养中SC之间的生物学差异，为细胞培养肉种子细胞的快速扩增和定制支架的开发提供了新的视角。
