Abstract：
In this article, the synthesis of antioxidant peptides in the enzymatic hydrolysis of caprine casein was analyzed at three different time points (60 min, 90 min, and 120 min) using immobilized pepsin on activated and modified carbon (AC, ACF, ACG 50, ACG 100). The immobilization assays revealed a reduction in the biocatalysts\' activity compared to the free enzyme. Among the modified ones, ACG 50 exhibited greater activity and better efficiency for reuse cycles, with superior values after 60 min and 90 min. Peptide synthesis was observed under all studied conditions. Analyses (DPPH, β-carotene/linoleic acid, FRAP) confirmed the antioxidant potential of the peptides generated by the immobilized enzyme. However, the immobilized enzyme in ACG 50 and ACG 100, combined with longer hydrolysis times, allowed the formation of peptides with an antioxidant capacity greater than or equivalent to those generated by the free enzyme, despite reduced enzymatic activity.
摘要：
在这篇文章中,在三个不同的时间点（60分钟，90分钟,和120分钟），使用固定化胃蛋白酶在活性炭和改性碳上（AC，ACF,ACG50，ACG100）。固定化试验表明，与游离酶相比，生物催化剂的活性降低。在修改的那些中，ACG50在重复使用周期中表现出更高的活性和更高的效率，60分钟和90分钟后具有优异的值。在所有研究条件下观察到肽合成。分析(DPPH，β-胡萝卜素/亚油酸,FRAP）证实了固定化酶产生的肽的抗氧化潜力。然而,ACG50和ACG100中的固定化酶，结合更长的水解时间，允许形成抗氧化能力大于或等于游离酶产生的肽，尽管酶活性降低。
